CPAP3 proteins in the mineralized cuticle of a decapod crustacean
文献类型：期刊论文
| 作者 | Abehsera, Shai1,2; Zaccai, Shir1; Mittelman, Binyamin1,2; Glazer, Lilah4; Weil, Simy1,2; Khalaila, Isam2,3; Davidov, Geula1,2; Bitton, Ronit1,2; Zarivach, Raz1,2; Li, Shihao5 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2018-02-05 |
| 卷号 | 8 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-20835-x |
| 文献子类 | Article |
| 英文摘要 | The pancrustacean theory groups crustaceans and hexapods (once thought to comprise separate clades within the Arthropoda) into a single clade. A key feature common to all pancrustaceans is their chitinous exoskeleton, with a major contribution by cuticular proteins. Among these, are the CPAP3' s, a family of cuticular proteins, first identified in the hexapod Drosophila melanogaster and characterized by an N-terminal signaling peptide and three chitin-binding domains. In this study, CPAP3 proteins were mined from a transcriptomic library of a decapod crustacean, the crayfish Cherax quadricarinatus. Phylogenetic analysis of other CPAP3 proteins from hexapods and other crustaceans showed a high degree of conservation. Characterization of the crayfish proteins, designated CqCPAP3's, suggested a major role for CPAP3'sin cuticle formation. Loss-of-function experiments using RNAi supported such a notion by demonstrating crucial roles for several CqCPAP3 proteins during molting. A putative mode of action for the CqCPAP3 proteins-theoretically binding three chitin strands-was suggested by the structural data obtained from a representative recombinant CqCPAP3. The similarities between the CqCPAP3 proteins and their hexapod homologues further demonstrated common genetic and proteinaceous features of cuticle formation in pancrustaceans, thereby reinforcing the linkage between these two highly important phylogenetic groups. |
| WOS关键词 | EXTRACELLULAR-MATRIX ; BIOLOGICAL-MATERIALS ; CALCIUM-TRANSPORT ; CHITIN-BINDING ; BOMBYX-MORI ; MOLT CYCLE ; CRAYFISH ; GASTROLITH ; DROSOPHILA ; ORGANIZATION |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000424087700102 |
| 出版者 | NATURE PUBLISHING GROUP |
| 资助机构 | Israel Science Foundation(613/13) ; Israel Science Foundation ; National Natural Science Foundation of China(605/14) ; National Institute for Biotechnology in the Negev (NIBN) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/10647]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Sagi, Amir |
| 作者单位 | 1.Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel 2.Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, Beer Sheva, Israel 3.Ben Gurion Univ Negev, Dept Biotechnol Engn, Beer Sheva, Israel 4.Duke Univ, Med Ctr, Dept Psychiat & Behav Sci, Durham, NC USA 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Abehsera, Shai,Zaccai, Shir,Mittelman, Binyamin,et al. CPAP3 proteins in the mineralized cuticle of a decapod crustacean[J]. SCIENTIFIC REPORTS,2018,8. |
| APA | Abehsera, Shai.,Zaccai, Shir.,Mittelman, Binyamin.,Glazer, Lilah.,Weil, Simy.,...&Sagi, Amir.(2018).CPAP3 proteins in the mineralized cuticle of a decapod crustacean.SCIENTIFIC REPORTS,8. |
| MLA | Abehsera, Shai,et al."CPAP3 proteins in the mineralized cuticle of a decapod crustacean".SCIENTIFIC REPORTS 8(2018). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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