MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis
文献类型：期刊论文
| 作者 | Li, Shengjun1,2,3; Liu, Kan2,3; Zhou, Bangjun2,4; Li, Mu2,3; Zhang, Shuxin5; Zeng, Lirong2,4; Zhang, Chi2,3; Yu, Bin2,3 |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 30期号:2页码:481-494 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.17.00953 |
| 文献子类 | Article |
| 英文摘要 | MAC3A and MAC3B are conserved U-box-containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis thaliana. However, their functional mechanisms remain elusive. Here, we show that Arabidopsis MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs (MIR genes), suggesting that they may not affect MIR transcription. This result, together with the fact that MAC3A associates with primiRNAs in vivo, indicates that MAC3A and MAC3B may stabilize pri-miRNAs. Furthermore, we find that MAC3A and MAC3B interact with the DCL1 complex that catalyzes miRNA maturation, promote DCL1 activity, and are required for the localization of HYL1, a component of the DCL1 complex. Besides MAC3A and MAC3B, two other MAC subunits, CDC5 and PRL1, also function in miRNA biogenesis. Based on these results, we propose that MAC functions as a complex to control miRNA levels through modulating pri-miRNA transcription, processing, and stability. |
| WOS关键词 | PRIMARY MICRORNA TRANSCRIPTS ; TRANS-ACTING SIRNAS ; BINDING PROTEIN ; ARABIDOPSIS-THALIANA ; SMALL RNAS ; PRI-MIRNA ; PLANT MICRORNAS ; CYCLOPHILIN 40 ; POLYMERASE-II ; U-BOX |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000427109700023 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | Nebraska Soybean Board(1727) ; National Science Foundation(OIA-1557417 ; Pioneer Hundred Talents Program of Chinese Academy of Sciences ; IOS-1645659) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/10711]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Yu, Bin |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Qingdao 266101, Peoples R China 2.Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA 3.Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA 4.Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA 5.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shengjun,Liu, Kan,Zhou, Bangjun,et al. MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis[J]. PLANT CELL,2018,30(2):481-494. |
| APA | Li, Shengjun.,Liu, Kan.,Zhou, Bangjun.,Li, Mu.,Zhang, Shuxin.,...&Yu, Bin.(2018).MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis.PLANT CELL,30(2),481-494. |
| MLA | Li, Shengjun,et al."MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis".PLANT CELL 30.2(2018):481-494. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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