Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress
文献类型：期刊论文
| 作者 | Zheng, Rikuan1,2,3; Wu, Shimei4; Ma, Ning1,2,3; Sun, Chaomin1,2 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2018-04-05 |
| 卷号 | 9 |
| 关键词 | Marine Pseudomonas Stutzeri Mercury Stress Motility Flagella |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2018.00682 |
| 文献子类 | Article |
| 英文摘要 | Mercury-mediated toxicity remains one of the greatest barriers against microbial survival, even though bacterial resistance to mercury compounds can occur. However, the genetic and physiological adaptations of bacteria to mercury stress still remains unclear. Here, we show that the marine bacterium Pseudomonas stutzeri 273 is resistant to 50 mu M Hg2+ and removes up to 94% Hg2+ from culture. Using gene homologous recombination and complementation, we show that genes encoding Hg2+-transport proteins MerT, MerP, the mercuric reductase MerA and the regulatory protein MerD are essential for bacterial mercuric resistance when challenged with Hg2+. Further, mercury stress inhibits flagellar development, motility, chemotaxis and biofilm formation of P. stutzeri 273, which are verified by transcriptomic and physiological analyses. Surprisingly, we discover that MerF, a previously reported Hg2+-transporter, determines flagellar development, motility and biofilm formation in P. stutzeri 273 by genetic and physiological analyses. Our results strongly indicate that MerF plays an integral role in P. stutzeri 273 to develop physiological responses to mercury stress. Notably, MerF homologs are also prevalent in different human pathogens. Using this unique target may provide novel strategies to control these pathogenic bacteria, given the role of MerF in flagella and biofilm formation. In summary, our data provide an original report on MerF in bacterial physiological development and suggest that the mer in marine bacteria has evolved through progressive, sequential recruitment of novel functions over time. |
| WOS关键词 | ESCHERICHIA-COLI ; SWARMING MOTILITY ; RESISTANCE OPERON ; MER OPERON ; PROTEIN ; TRANSPORT ; BIOREMEDIATION ; DETOXIFICATION ; PRECIPITATION ; MECHANISM |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000429289200001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | Taishan Young Scholar Foundation of Shandong Province(tsqn20161051) ; Qingdao National Laboratory for Marine Science and Technology(2015ASTP) ; Natural Science Outstanding Youth Fund of Shandong Province(JQ201607) ; "100-Talent Project" of the Chinese Academy of Sciences |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/10727]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Sun, Chaomin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 4.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Rikuan,Wu, Shimei,Ma, Ning,et al. Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress[J]. FRONTIERS IN MICROBIOLOGY,2018,9. |
| APA | Zheng, Rikuan,Wu, Shimei,Ma, Ning,&Sun, Chaomin.(2018).Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress.FRONTIERS IN MICROBIOLOGY,9. |
| MLA | Zheng, Rikuan,et al."Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress".FRONTIERS IN MICROBIOLOGY 9(2018). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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