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The molecular basis for lipase stereoselectivity

文献类型：期刊论文

作者Chen, Hui1; Meng, Xiao2; Xu, Xiaoqing3; Liu, Wenbo3; Li, Shengying1
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2018-04-01
卷号102期号:8页码:3487-3495
关键词Lipase Stereoselectivity Steric Exclusion Structural Flexibility Hydrogen Bond Electrostatic Interaction
ISSN号0175-7598
DOI10.1007/s00253-018-8858-z
文献子类Review
英文摘要Lipases are among the most applied biocatalysts in organic synthesis to catalyze the kinetic resolution of a wide range of racemic substrates to yield optically pure compounds. Due to the rapidly increased demands for optically pure compounds, deep understanding of the molecular basis for lipase stereoselectivity and how to obtain lipases with excellent asymmetric selectivity have become one of primary research goals in this field. This review is focused on the molecular factors that have impacts on the stereoselectivity of lipases including the steric complementarity between the lipase topological structure and its substrate, the regional structural flexibility, the hydrogen bonds between the residues around the catalytic site and the tetrahedral intermediates, and the electrostatic interactions between surface residues. Moreover, the synergistic effects of these structural factors on the catalytic properties including stereoselectivity, activity, and stability are also discussed.
WOS关键词CANDIDA-RUGOSA LIPASE ; PSEUDOMONAS-CEPACIA LIPASE ; YARROWIA-LIPOLYTICA LIPASE ; SITE-DIRECTED MUTAGENESIS ; RHIZOMUCOR-MIEHEI LIPASE ; SUBSTRATE-BINDING SITE ; PATH-PLANNING APPROACH ; ORGANIC-SOLVENTS ; CHEMICAL-MODIFICATION ; SECONDARY ALCOHOLS
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000428316700004
出版者SPRINGER
资助机构National Natural Science Foundation of China(NSFC 21406250) ; Applied Basic Research Programs of Science and Technology of Qingdao(15-9-1-106-jch)
源URL[http://ir.qibebt.ac.cn/handle/337004/10743]  
专题青岛生物能源与过程研究所_酶工程团队
通讯作者Li, Shengying
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, CAS Key Lab Biofuels, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China
2.Shanghai SynTheAll Pharmaceut Co Ltd, 90 Delin Rd Waigaoqiao Pilot Free Trade, Shanghai 200131, Peoples R China
3.Rushan Hanwei Biol Sci & Technol Co Ltd, 306 Qingshan Rd, Rushan 264502, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Hui,Meng, Xiao,Xu, Xiaoqing,et al. The molecular basis for lipase stereoselectivity[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2018,102(8):3487-3495.
APA Chen, Hui,Meng, Xiao,Xu, Xiaoqing,Liu, Wenbo,&Li, Shengying.(2018).The molecular basis for lipase stereoselectivity.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,102(8),3487-3495.
MLA Chen, Hui,et al."The molecular basis for lipase stereoselectivity".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.8(2018):3487-3495.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

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