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Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts

文献类型：期刊论文

作者Zhang, Ning1,2; Fan, Yuxuan1,2; Li, Chen3,4; Wang, Qiming5; Leksawasdi, Noppol6; Li, Fuli1; Wang, Shi'an1
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2018-05-01
卷号102期号:9页码:4183-4191
关键词Basidiomycetous Yeast Cell Permeability Cell Wall Dimethyl Sulfoxide Dna Staining
ISSN号0175-7598
DOI10.1007/s00253-018-8906-8
文献子类Article
英文摘要Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in physiology, ecology, cell biology, and genetics. However, it is unclear whether the classical nuclear DNA staining method for ascomycetous yeasts is applicable to basidiomycetous yeasts. In this study, 5 yeasts ineffectively stained by the classical propidium iodide (PI) staining method were identified from 23 representative basidiomycetous yeasts. Pretreatment of cells using dimethyl sulfoxide (DMSO) or snailase markedly improved cell penetration to PI and thus enabled DNA content determination by flow cytometry on the recalcitrant yeasts. The pretreatments are efficient, simple, and fast, avoiding tedious mutagenesis or genetic engineering used in previous reports. The heterogeneity of cell penetration to PI among basidiomycetous yeasts was attributed to the discrepancy in cell wall polysaccharides instead of capsule or plasma membrane. This study also indicated that care must be taken in attributing PI-negative staining as viable cells when studying non-model microorganisms.
WOS关键词SACCHAROMYCES-CEREVISIAE ; PHAFFIA-RHODOZYMA ; FLOW-CYTOMETRY ; DIMETHYL-SULFOXIDE ; PHYLOGENETIC CLASSIFICATION ; CRYPTOCOCCUS-NEOFORMANS ; PENETRATION ENHANCERS ; ESCHERICHIA-COLI ; WALL ; VIABILITY
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000429800600026
出版者SPRINGER
资助机构Natural Science Foundation of China(31670054 ; Youth Innovation Promotion Association, Chinese Academy of Sciences(2013137) ; 51561145015 ; 21676159)
源URL[http://ir.qibebt.ac.cn/handle/337004/10767]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Wang, Shi'an
作者单位1.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Qingdao Engn Lab Single Cell Oil, Key Lab Biofuels,Qingdao Inst Bioenergy & Bioproc, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Chinese Acad Fishery Sci, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Key Lab Maricultural Organism Dis Control, Minist Agr,Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China
6.Chiang Mai Univ, Sch Agroind, Chiang Mai 50100, Thailand
推荐引用方式
GB/T 7714		 Zhang, Ning,Fan, Yuxuan,Li, Chen,et al. Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2018,102(9):4183-4191.
APA Zhang, Ning.,Fan, Yuxuan.,Li, Chen.,Wang, Qiming.,Leksawasdi, Noppol.,...&Wang, Shi'an.(2018).Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,102(9),4183-4191.
MLA Zhang, Ning,et al."Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.9(2018):4183-4191.

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来源：青岛生物能源与过程研究所

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