Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress
文献类型：期刊论文
| 作者 | Ma, Zengxin1,4,5; Tian, Miaomiao2; Tan, Yanzhen1,6; Cui, Guzhen1,4 ; Feng, Yingang1,4; Cui, Qiu1,3,4; Song, Xiaojin1,4
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| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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| 出版日期 | 2017-07-01 |
| 卷号 | 25页码:191-199 |
| 关键词 | Aurantiochytrium Cold Stress Dha 2d Gels Itraq Proteome |
| DOI | 10.1016/j.algal.2017.05.021 |
| 文献子类 | Article |
| 英文摘要 | Aurantiochytrium is a commercial docosahexaenoic acid (DHA) producer, and its DHA content can be significantly increased under cold stress. Given this response to low temperature, we examined proteomics changes in Aurantiochytrium under cold stress. We detected approximately 700 protein spots using twodimensional gels, whereas using iTRAQ technology, we detected 4650 types of proteins and successfully identified> 53%. The results indicated that cold stress inhibits the cellular energy supply from glycolysis and the TCA cycle, to ensure a sufficient supply of NADPH and ribose for anabolism. In contrast, the pentose phosphate pathway was not affected. With respect to lipid synthesis, low temperature led to a significant downregulation and up-regulation of fatty acid synthase and polyunsaturated fatty acid synthase, respectively, and restricted the protein synthesis of diacylglycerol O-acyltransferase and phospholipid: diacylglycerol acyltransferase. These results show the preferential biosynthesis of polyunsaturated fatty acids and phospholipids by Aurantiochytrium, which collectively serve to increase cell survival rates in cold environments. |
| WOS关键词 | FATTY-ACIDS ; SCHIZOCHYTRIUM SP ; METABOLOMICS ANALYSIS ; PROTEOMIC ANALYSIS ; ANALYSIS REVEALS ; TEMPERATURE ; PROTEIN ; TRANSCRIPTOME ; ACCUMULATION ; GROWTH |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000406305900023 |
| 资助机构 | National Key Research and Development Program(2016YFA0601400) ; National Natural Science Foundation of China(41306132 ; National High Technology Research and Development Program of China(2014AA021701) ; Qingdao Science and Technology Development Plan(14-2-4-69-jch) ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation for Young Scientists(QIBEBT-201503) ; 31500078) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9549]  |
| 专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp Digest Dis, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China 4.Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Shandong, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Fourth Mil Med Univ, Xijing Hosp, Dept Cardiovasc Surg, Xian 710032, Shaanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Zengxin,Tian, Miaomiao,Tan, Yanzhen,et al. Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2017,25:191-199. |
| APA | Ma, Zengxin.,Tian, Miaomiao.,Tan, Yanzhen.,Cui, Guzhen.,Feng, Yingang.,...&Song, Xiaojin.(2017).Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,25,191-199. |
| MLA | Ma, Zengxin,et al."Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 25(2017):191-199. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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