Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases
文献类型：期刊论文
| 作者 | Wang, Hui1,2; Gao, Lili1,2 ; Shao, Huimin1,3; Zhou, Wenjun1,2; Liu, Tianzhong1,2
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| 刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| 出版日期 | 2017-09-01 |
| 卷号 | 40期号:9页码:1327-1335 |
| 关键词 | Tribonema Minus Confocal Image Lipid Synthesis Transcriptome Sequencing Differential Expression |
| DOI | 10.1007/s00449-017-1791-1 |
| 文献子类 | Article |
| 英文摘要 | Filamentous oleaginous microalgae specie Tribonema minus is a promising feedstock for biodiesel production. However, the metabolic mechanism of lipid production in this filamentous microalgal specie remains unclear. Here, we compared the lipid accumulation of T. minus at different growth phases, and described the de novo transcriptome sequencing and assembly and identified important pathways and genes involved in TAG production. Total lipid increased by 2.5-fold and its TAG level in total lipid reached 81.1% at stationary phase. Using the genes involved in the lipid metabolism, the TAG biosynthesis pathways were generated. Moreover, results also demonstrated that, in addition to the observed overexpression of the fatty acid synthesis pathway, TAG production at stationary growth phase was bolstered by repression of the beta-oxidation pathway, up-regulation of genes that funnels acetyl-CoA to lipid biosynthesis, especially gene encoding for phospholipid:diacylglycerol acyltransferase (PDAT) which funnels DAG to TAG biosynthesis. |
| WOS关键词 | CHLORELLA MINUTISSIMA ; BIODIESEL PRODUCTION ; BIOFUEL PRODUCTION ; MASS-SPECTROMETRY ; TRIACYLGLYCEROLS ; PERSPECTIVES ; ANNOTATION ; STRAIN ; GENES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000407997600004 |
| 资助机构 | Research Program of Application Foundation of Qingdao from Qingdao Science and Technology Bureau(16-5-1-68-jch) ; National Key R&D program from Ministry of Science and Technology of China(2016YFB0601001-02) ; Marine economic innovation and development regional demonstration project of Qingdao |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9555]  |
| 专题 | 青岛生物能源与过程研究所_微藻生物技术团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Bioenergy Resources, Qingdao 266101, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hui,Gao, Lili,Shao, Huimin,et al. Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2017,40(9):1327-1335. |
| APA | Wang, Hui,Gao, Lili,Shao, Huimin,Zhou, Wenjun,&Liu, Tianzhong.(2017).Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases.BIOPROCESS AND BIOSYSTEMS ENGINEERING,40(9),1327-1335. |
| MLA | Wang, Hui,et al."Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases".BIOPROCESS AND BIOSYSTEMS ENGINEERING 40.9(2017):1327-1335. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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