Backbone and side-chain H-1, N-15 and C-13 resonance assignments of two Sac10b family members Mvo10b and Mth10bTQQA from archaea
文献类型：期刊论文
| 作者 | Xuan, Jinsong1; Yao, Hongwei2; Feng, Yingang3 ; Wang, Jinfeng4
|
| 刊名 | BIOMOLECULAR NMR ASSIGNMENTS
 |
| 出版日期 | 2017-10-01 |
| 卷号 | 11期号:2页码:269-273 |
| 关键词 | Sac10b Family Mesophilic Thermal Adaptation Mechanism Nmr Assignments Archaea |
| DOI | 10.1007/s12104-017-9761-8 |
| 文献子类 | Article |
| 英文摘要 | The Sac10b family proteins, also named as Alba, are small, basic, nucleic acid-binding proteins widely distributed in archaea. They possess divergent physiological functions such as binding to both DNA and RNA with a high affinity and involving in genomic DNA compaction, RNA transactions and transcriptional regulations. The structures of many Sac10b family proteins from hyperthermophilic archaea have been reported, while those from thermophilic and mesophilic archaea are largely unknown. As was pointed out, the homologous members from thermophilic and mesophilic archaea may have functions different from the hyperthermophilic members. Therefore, comparison of these homologous members can provide biophysical and structural insight into the functional diversity and thermal adaptation mechanism. The present work mainly focused on the NMR study of two Sac10b family members, Mvo10b and Mth10b, from the mesophilic and thermophilic archaea, respectively. To overcome the difficulties caused by the oligomerization and conformation heterogeneity of Mth10b, a M13T/L17Q/I20Q/P56A mutant Mth10b (Mth10bTQQA) was constructed and used together with Mvo10b for multi-dimensional NMR experiments. The resonance assignments of Mvo10b and Mth10bTQQA are reported for further structural determination which is a basis for understanding the functional diversity and their thermal adaption mechanisms. |
| WOS关键词 | PROTEIN FAMILY ; CHROMATIN ; DNA ; BINDING ; SSH10B ; ALBA |
| WOS研究方向 | Biophysics ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000410473300029 |
| 资助机构 | National Natural Science Foundation of China(31300635 ; 31670735 ; 30770434) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9579]  |
| 专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Dept Biol Sci & Engn, 30 Xueyuan Rd, Beijing 100083, Peoples R China 2.Xiamen Univ, High Field Nucl Magnet Resonance Res Ctr, 422 South Siming Rd, Xiamen 361005, Fujian, Peoples R China 3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xuan, Jinsong,Yao, Hongwei,Feng, Yingang,et al. Backbone and side-chain H-1, N-15 and C-13 resonance assignments of two Sac10b family members Mvo10b and Mth10bTQQA from archaea[J]. BIOMOLECULAR NMR ASSIGNMENTS,2017,11(2):269-273. |
| APA | Xuan, Jinsong,Yao, Hongwei,Feng, Yingang,&Wang, Jinfeng.(2017).Backbone and side-chain H-1, N-15 and C-13 resonance assignments of two Sac10b family members Mvo10b and Mth10bTQQA from archaea.BIOMOLECULAR NMR ASSIGNMENTS,11(2),269-273. |
| MLA | Xuan, Jinsong,et al."Backbone and side-chain H-1, N-15 and C-13 resonance assignments of two Sac10b family members Mvo10b and Mth10bTQQA from archaea".BIOMOLECULAR NMR ASSIGNMENTS 11.2(2017):269-273. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。