Biochemical Characterization and Substrate Degradation Mode of a Novel Exotype beta-Agarase from Agarivorans gilvus WHO801
文献类型：期刊论文
| 作者 | Liang, Yunxiao1; Ma, Xiaoqing2 ; Zhang, Lujia1,3; Li, Fuli2; Liu, Zhen1; Mao, Xiangzhao1
|
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2017-09-13 |
| 卷号 | 65期号:36页码:7982-7988 |
| 关键词 | Agarase Ghso Family Agarivorans Gilvus Degradation Mode |
| DOI | 10.1021/acs.jafc7b01533 |
| 文献子类 | Article |
| 英文摘要 | Agarases are important hydrolytic enzymes for the biodegradation of agar. Understanding the degradation mode and hydrolysis products of agarases is essential for their utilization in oligosaccharide preparations. Herein, we cloned and expressed AgW1150B, a novel neoagarotetraose-forming beta-agarase from Agarivorans gilvus WHO801 that has high specific activity and a fast reaction rate. AgW1150B consists of a C-terminal glycoside hydrolase family 50 catalytic domain with two tandem noncatalytic carbohydrate-binding modules (CBMs) in the N-terminus (residues 45-214 and 236-442). AgWHSOB exhibited good enzymatic properties with high specific activity and catalytic efficiency (1523.2 U/mg and a V-max of 1700 mu mol/min/mg) under optimal hydrolysis conditions of pH 7.0 and 40 degrees C. Analysis of the hydrolysis products revealed that this enzyme is an exotype beta-agarase and that the dominant product of agarose or oligosaccharide degradation was neoagarotetraose. These findings suggest that AgVVH5OB could be utilized to yield abundant neoagarotetraose. |
| WOS关键词 | MARINE BACTERIUM ; SACCHAROPHAGUS-DEGRADANS ; GENE CLONING ; ENZYMATIC-HYDROLYSIS ; ALPHA-AGARASE ; ALBUS YKW-34 ; PURIFICATION ; EXPRESSION ; NEOAGAROBIOSE ; OLIGOSACCHARIDES |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000411043800017 |
| 资助机构 | National Natural Science Foundation of China(31471607 ; China Postdoctoral Science Foundation(2016M590661) ; 31271923) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9584]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 作者单位 | 1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Yunxiao,Ma, Xiaoqing,Zhang, Lujia,et al. Biochemical Characterization and Substrate Degradation Mode of a Novel Exotype beta-Agarase from Agarivorans gilvus WHO801[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(36):7982-7988. |
| APA | Liang, Yunxiao,Ma, Xiaoqing,Zhang, Lujia,Li, Fuli,Liu, Zhen,&Mao, Xiangzhao.(2017).Biochemical Characterization and Substrate Degradation Mode of a Novel Exotype beta-Agarase from Agarivorans gilvus WHO801.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(36),7982-7988. |
| MLA | Liang, Yunxiao,et al."Biochemical Characterization and Substrate Degradation Mode of a Novel Exotype beta-Agarase from Agarivorans gilvus WHO801".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.36(2017):7982-7988. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。