Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides
文献类型:期刊论文
作者 | Abu Saleh, Mohammad1,2,3; Han, Wen-Jie1,2,4; Lu, Ming1,2; Wang, Bing1,2; Li, Huayue5; Kelly, Robert M.6; Li, Fu-Li1,2 |
刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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出版日期 | 2017-07-01 |
卷号 | 83期号:13 |
关键词 | Arabinofuranosidase Bioenergy Glycoside Hydrolase Hyperthermophiles Synergism |
DOI | 10.1128/AEM.00574-17 |
文献子类 | Article |
英文摘要 | Species in the extremely thermophilic genus Caldicellulosiruptor can degrade unpretreated plant biomass through the action of multimodular glycoside hydrolases. To date, most focus with these bacteria has been on hydrolysis of glucans and xylans, while the biodegradation mechanism for arabinose-based polysaccharides remains unclear. Here, putative alpha-L-arabinofuranosidases (AbFs) were identified in Caldicellulosiruptor species by homology to less-thermophilic versions of these enzymes. From this screen, an extracellular XynF was determined to be a key factor in hydrolyzing alpha-1,2-, alpha-1,3-, and alpha-1,5-L-arabinofuranosyl residues of arabinose-based polysaccharides. Combined with a GH11 xylanase (XynA), XynF increased arabinoxylan hydrolysis more than 6-fold compared to the level seen with XynA alone, likely the result of XynF removing arabinofuranosyl side chains to generate linear xylans that were readily degraded. A second AbF, the intracellular AbF51, preferentially cleaved the alpha-1,5-L-arabinofuranosyl glycoside bonds within sugar beet arabinan. beta-Xylosidases, such as GH39 Xyl39B, facilitated the hydrolysis of arabinofuranosyl residues at the nonreducing terminus of the arabinose-branched xylo-oligosaccharides by AbF51. These results demonstrate the separate but complementary contributions of extracellular XynF and cytosolic AbF51 in processing the bioconversion of arabinose-containing oligosaccharides to fer-mentable monosaccharides. |
WOS关键词 | FREE QUANTITATIVE PROTEOMICS ; GLYCOSIDE HYDROLASE ; PLANT BIOMASS ; INTESTINAL SUCRASE ; GH11 XYLANASES ; DSM 6725 ; SP F32 ; SPECIFICITY ; DIVERSITY ; CELLULOSE |
WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
语种 | 英语 |
WOS记录号 | WOS:000403495700015 |
资助机构 | National Natural Science Foundation of China(31400060) ; Shandong Province Natural Science Funds for Distinguished Young Scholar(JQ201507) ; Key Scientific and Technological Project of Shandong Province(2015ZDXX0403A01) |
源URL | [http://ir.qibebt.ac.cn/handle/337004/9640] ![]() |
专题 | 青岛生物能源与过程研究所_微生物资源团队 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao Engn Lab Single Cell Oil,Key Lab Biofuel, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Rajshahi, Dept Genet Engn & Biotechnol, Rajshahi, Bangladesh 4.Qingdao Univ, Coll Environm Sci & Engn, Qingdao, Peoples R China 5.Ocean Univ China, Sch Med & Pharm, Qingdao, Peoples R China 6.North Carolina State Univ, Dept Chem & Biomol Engn, Raleigh, NC USA |
推荐引用方式 GB/T 7714 | Abu Saleh, Mohammad,Han, Wen-Jie,Lu, Ming,et al. Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(13). |
APA | Abu Saleh, Mohammad.,Han, Wen-Jie.,Lu, Ming.,Wang, Bing.,Li, Huayue.,...&Li, Fu-Li.(2017).Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(13). |
MLA | Abu Saleh, Mohammad,et al."Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.13(2017). |
入库方式: OAI收割
来源:青岛生物能源与过程研究所
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