中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 青岛生物能源与过程研究所
  3. 中国科学院青岛生物能源与过程研究所
  4. 酶工程团队
Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system

文献类型：期刊论文

作者Du, Lei1,2,3; Dong, Sheng1,2; Zhang, Xingwang1,2; Jiang, Chengying4,5; Chen, Jingfei1,2; Yao, Lishan1,2; Wang, Xiao6; Wan, Xiaobo6; Liu, Xi7,8,9; Wang, Xinquan7,8,9
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2017-06-27
卷号114期号:26页码:E5129-E5137
关键词Alkylphenol Selective Oxidation Chemomimetic Biocatalysis Cytochrome P450 Enzymes Corynebacterium Glutamicum
DOI10.1073/pnas.1702317114
文献子类Article
英文摘要Selective oxidation of aliphatic C-H bonds in alkylphenols serves significant roles not only in generation of functionalized intermediates that can be used to synthesize diverse downstream chemical products, but also in biological degradation of these environmentally hazardous compounds. Chemo-, regio-, and stereoselectivity; controllability; and environmental impact represent the major challenges for chemical oxidation of alkylphenols. Here, we report the development of a unique chemomimetic biocatalytic system originated from the Gram-positive bacterium Corynebacterium glutamicum. The system consisting of CreHI (for installation of a phosphate directing/anchoring group), CreJEF/CreG/CreC (for oxidation of alkylphenols), and CreD (for directing/anchoring group offloading) is able to selectively oxidize the aliphatic C-H bonds of p-and m-alkylated phenols in a controllable manner. Moreover, the crystal structures of the central P450 biocatalyst CreJ in complex with two representative substrates provide significant structural insights into its substrate flexibility and reaction selectivity.
WOS关键词XYLENE MONOOXYGENASE ; CYTOCHROME-P450 ENZYMES ; SUBSTRATE RECOGNITION ; MOLECULAR RECOGNITION ; BACILLUS-SUBTILIS ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; DIRECTING GROUP ; HYDROXYLATION ; OXYGENATION
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000404108400016
资助机构National Natural Science Foundation of China(NSFC 31422002 ; Shandong Provincial Natural Science Foundation(JQ201407) ; Key Frontier Project of the Chinese Academy of Sciences(QYZDB-SSW-SMC042) ; 973 Project from Ministry of Science and Technology(2012CB7211-04) ; NSFC 31270784)
源URL[http://ir.qibebt.ac.cn/handle/337004/9645]  
专题青岛生物能源与过程研究所_酶工程团队
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
5.Chinese Acad Sci, Inst Microbiol, Environm Microbiol & Biotechnol Res Ctr, Beijing 100101, Peoples R China
6.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Shandong, Peoples R China
7.Tsinghua Univ, Sch Life Sci, Key Lab Prot Sci, Minist Educ, Beijing 100084, Peoples R China
8.Tsinghua Univ, Sch Life Sci, Beijing Adv Innovat Ctr Struct Biol, Beijing 100084, Peoples R China
9.Tsinghua Univ, Sch Life Sci, Collaborat Innovat Ctr Biotherapy, Beijing 100084, Peoples R China
推荐引用方式
GB/T 7714		 Du, Lei,Dong, Sheng,Zhang, Xingwang,et al. Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(26):E5129-E5137.
APA Du, Lei.,Dong, Sheng.,Zhang, Xingwang.,Jiang, Chengying.,Chen, Jingfei.,...&Li, Shengying.(2017).Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(26),E5129-E5137.
MLA Du, Lei,et al."Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.26(2017):E5129-E5137.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。