Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application
文献类型：期刊论文
| 作者 | Zhao, Fuhua1,2; Wang, Qiao1,2; Dong, Jianjun1; Xian, Mo2; Yu, Junhong1; Yin, Hua1; Chang, Zongming1; Mu, Xindong2; Hou, Tonggang2; Wang, Jianxun2 |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2017-06-01 |
| 卷号 | 57页码:87-94 |
| 关键词 | Enzyme-inorganic Nanoflowers Microbeads Alginate Enzyme Immobilization Diacetyl |
| DOI | 10.1016/j.procbio.2017.03.026 |
| 文献子类 | Article |
| 英文摘要 | In this work, novel nanoflower/alginate microbeads were synthesized to immobilize alpha-acetolactate decarboxylase (ALDC) with a facile approach. Typically, ALDC was first coprecipitated with Ca-3(PO4)(2) to form enzyme inorganic hybrid nanoflowers (Ca-3(PO4)(2)-ALDC), and then the nanoflowers containing micrometre-sized particles and nanoscale flower-like petals were entrapped in alginate gel beads (Ca-3(PO4)(2)-ALDC)@Mg). Compared with free ALDC and Ca-3(PO4)(2)-ALDC nanoflowers, the microbeads performed greatly improved recyclability and stability, meanwhile, retained 98% of activity compared with free ALDC. Moreover, the immobilized enzyme can be used in a 300 L beer fermentation tank to prevent the formation of diacetyl, solve the problem of beer off-flavor and short the beer maturation time, thus exhibiting a great potential application in beer brewing industry. |
| WOS关键词 | ALPHA-ACETOLACTATE DECARBOXYLASE ; CA-ALGINATE BEADS ; HYBRID NANOFLOWERS ; GRAPHENE OXIDE ; EFFICIENT METHOD ; ENCAPSULATION ; LIPASE ; MICROCAPSULES ; ENTRAPMENT ; CAPSULES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000403121600012 |
| 资助机构 | National Natural Science Foundation of China(21433001 ; Applied Basic Research Foundation of Qingdao City(14-2-4-21-jch) ; 21603251) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9650]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Tsingtao Brewery Co Ltd, State Key Lab Biol Fermentat Engn Beer, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Fuhua,Wang, Qiao,Dong, Jianjun,et al. Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application[J]. PROCESS BIOCHEMISTRY,2017,57:87-94. |
| APA | Zhao, Fuhua.,Wang, Qiao.,Dong, Jianjun.,Xian, Mo.,Yu, Junhong.,...&Wang, Jianxun.(2017).Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application.PROCESS BIOCHEMISTRY,57,87-94. |
| MLA | Zhao, Fuhua,et al."Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application".PROCESS BIOCHEMISTRY 57(2017):87-94. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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