Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death
文献类型：期刊论文
| 作者 | Liu, Sijia1,2; Fu, Chunxiang3 ; Gou, Jiqing2,4; Sun, Liang5; Huhman, David6; Zhang, Yunwei1; Wang, Zeng-Yu2,4
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| 刊名 | FRONTIERS IN PLANT SCIENCE
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| 出版日期 | 2017-06-20 |
| 卷号 | 8 |
| 关键词 | Bioenergy Crop Caffeic Acid O-methyltransferase (Comt) Lesion-mimic Cell Death Lignin Methylenetetrahydrofolate Reductase (Mthfr) Panicum Virgatum Switchgrass Transgenic Plants |
| DOI | 10.3389/fpls.2017.00982 |
| 文献子类 | Article |
| 英文摘要 | Switchgrass (Panicum virgatum) has been developed into a model lignocellulosic bioenergy crop. Downregulation of caffeic acid O-methyltransferase (COMT), a key enzyme in lignin biosynthesis, has been shown to alter lignification and increase biofuel yield in switchgrass. Methylenetetrahydrofolate reductase (MTHFR) mediates C1 metabolism and provides methyl units consumed by COMT. It was predicted that co-silencing of MTHFR and COMT would impact lignification even more than either of the single genes. However, our results showed that strong downregulation of MTHFR in a COMT-deficient background led to altered plant growth and development, but no significant change in lignin content or composition was found when compared with COMT plants. Another unexpected finding was that the double MTHFR/COMT downregulated plants showed a novel lesion-mimic leaf phenotype. Molecular analyses revealed that the lesion-mimic phenotype was caused by the synergistic effect of MTHFR and COMT genes, with MTHFR playing a predominant role. Microarray analysis showed significant induction of genes related to oxidative and defense responses. The results demonstrated the lack of additive effects of MTHFR and COMT on lignification. Furthermore, this research revealed an unexpected role of the two genes in the modulation of lesion-mimic cell death as well as their synergistic effects on agronomic performance. |
| WOS关键词 | ACID O-METHYLTRANSFERASE ; BROWN-MIDRIB MUTANTS ; MODIFIED TOBACCO REVEALS ; METHYLENETETRAHYDROFOLATE REDUCTASE ; LIGNIN BIOSYNTHESIS ; PRIMARY METABOLISM ; DEFENSE RESPONSE ; FIELD ANALYSIS ; GENE ENCODES ; MAIZE |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000403638700001 |
| 资助机构 | China Scholarship Council ; BioEnergy Science Center ; Samuel Roberts Noble Foundation ; Ministry of Science and Technology, PR China(2014BAD23B03-02 ; 2012AA101801) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9712]  |
| 专题 | 青岛生物能源与过程研究所_作物分子育种团队 |
| 作者单位 | 1.China Agr Univ, Dept Grassland Sci, Natl Energy R&D Ctr Biomass, Beijing, Peoples R China 2.Samuel Roberts Noble Fdn Inc, Forage Improvement Div, Ardmore, OK 73401 USA 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 4.US DOE, Oak Ridge Natl Lab, BioEnergy Sci Ctr, Oak Ridge, TN 37830 USA 5.Samuel Roberts Noble Fdn Inc, Comp Serv, Ardmore, OK USA 6.Samuel Roberts Noble Fdn Inc, Plant Biol Div, Ardmore, OK USA |
| 推荐引用方式 GB/T 7714 | Liu, Sijia,Fu, Chunxiang,Gou, Jiqing,et al. Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death[J]. FRONTIERS IN PLANT SCIENCE,2017,8. |
| APA | Liu, Sijia.,Fu, Chunxiang.,Gou, Jiqing.,Sun, Liang.,Huhman, David.,...&Wang, Zeng-Yu.(2017).Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death.FRONTIERS IN PLANT SCIENCE,8. |
| MLA | Liu, Sijia,et al."Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death".FRONTIERS IN PLANT SCIENCE 8(2017). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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