Modulation of the Acetone/Butanol Ratio during Fermentation of Corn Stover-derived Hydrolysate by Clostridium beijerinckii NCIMB 8052
文献类型：期刊论文
| 作者 | Zi-Yong Liu; Xiu-Qing Yao; Quan Zhang; Zhen Liu; Ze-Jie Wang; Yong-Yu Zhang; Fu-Li Li |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2017-01-25 |
| 卷号 | 83期号:7页码:e03386-16 |
| 关键词 | Corn Stover Soluble Lignin Compounds Nadh/nad Ratio A/b Ratio Clostridium |
| DOI | 10.1128/ AEM.03386-16 |
| 产权排序 | 2 |
| 文献子类 | Article |
| 英文摘要 | Producing biobutanol from lignocellulosic biomass has shown promise to ultimately reduce greenhouse gases and alleviate the global energy crisis. However, because of the recalcitrance of a lignocellulosic biomass, a pretreatment of the substrate is needed which in many cases releases soluble lignin compounds (SLCs), which inhibit growth of butanol-producing clostridia. In this study, we found that SLCs changed the acetone/butanol ratio (A/B ratio) during butanol fermentation. The typical A/B molar ratio during Clostridium beijerinckii NCIMB 8052 batch fermentation with glucose as the carbon source is about 0.5. In the present study, the A/B molar ratio during batch fermentation with a lignocellulosic hydrolysate as the carbon source was 0.95 at the end of fermentation. Structural and redox potential changes of the SLCs were characterized before and after fermentation by using gas chromatography/mass spectrometry and electrochemical analyses, which indicated that some exogenous SLCs were involved in distributing electron flow to C. beijerinckii, leading to modulation of the redox balance. This was further demonstrated by the NADH/NAD ratio and trxB gene expression profile assays at the onset of solventogenic growth. As a result, the A/B ratio of end products changed significantly during C. beijerinckii fermentation using corn stover-derived hydrolysate as the carbon source compared to glucose as the carbon source. These results revealed that SLCs not only inhibited cell growth but also modulated the A/B ratio during C. beijerinckii butanol fermentation. |
| 学科主题 | 微生物 |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| 资助机构 | Shandong Province Natural Science Funds for Distinguished Young Scholar (JQ201507) ; National Natural Science Foundation of China (51561145015 and 30970049) ; China Petrochemical Corporation (Sinopec) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9838]  |
| 专题 | 青岛生物能源与过程研究所_海洋生物与碳汇研究中心 |
| 作者单位 | 1.School of Resources and Civil Engineering, Northeastern University 2.Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences 3.College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning University of Petroleum and Chemical Technology 4.Sinopec Fushun Research Institute of Petroleum and Petrochemicals |
| 推荐引用方式 GB/T 7714 | Zi-Yong Liu,Xiu-Qing Yao,Quan Zhang,et al. Modulation of the Acetone/Butanol Ratio during Fermentation of Corn Stover-derived Hydrolysate by Clostridium beijerinckii NCIMB 8052[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(7):e03386-16. |
| APA | Zi-Yong Liu.,Xiu-Qing Yao.,Quan Zhang.,Zhen Liu.,Ze-Jie Wang.,...&Fu-Li Li.(2017).Modulation of the Acetone/Butanol Ratio during Fermentation of Corn Stover-derived Hydrolysate by Clostridium beijerinckii NCIMB 8052.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(7),e03386-16. |
| MLA | Zi-Yong Liu,et al."Modulation of the Acetone/Butanol Ratio during Fermentation of Corn Stover-derived Hydrolysate by Clostridium beijerinckii NCIMB 8052".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.7(2017):e03386-16. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。