Using Raman spectroscopy and chemometrics to identify the growth phase of Lactobacillus casei Zhang during batch culture at the single-cell level.
文献类型：期刊论文
| 作者 | Yan Ren, Yuetong Ji, Lin Teng, Heping Zhang; Heping Zhang |
| 刊名 | Microbial Cell Factories
 |
| 出版日期 | 2017-12-23 |
| 卷号 | 16期号:1页码:233-243 |
| 关键词 | Lactobacillus Casei Zhang Growth Phases Single-cell Raman Spectrometry Chemometrics |
| DOI | 10.1186/s12934-017-0849-8 |
| 文献子类 | Article |
| 英文摘要 | Background As microbial cultures are comprised of heterogeneous cells that differ according to their size and intracellular concentrations of DNA, proteins, and other constituents, the detailed identification and discrimination of the growth phases of bacterial populations in batch culture is challenging. Cell analysis is indispensable for quality control and cell enrichment. Methods In this paper, we report the results of our investigation on the use of single-cell Raman spectrometry (SCRS) for real-time analysis and prediction of cells in different growth phases during batch culture of Lactobacillus (L.) casei Zhang. A targeted analysis of defined cell growth phases at the level of the single cell, including lag phase, log phase, and stationary phase, was facilitated by SCRS. Results Spectral shifts were identified in different states of cell growth that reflect biochemical changes specific to each cell growth phase. Raman peaks associated with DNA and RNA displayed a decrease in intensity over time, whereas protein-specific and lipid-specific Raman vibrations increased at different rates. Furthermore, a supervised classification model (Random Forest) was used to specify the lag phase, log phase, and stationary phase of cells based on SCRS, and a mean sensitivity of 90.7% and mean specificity of 90.8% were achieved. In addition, the correct cell type was predicted at an accuracy of approximately 91.2%. Conclusions To conclude, Raman spectroscopy allows label-free, continuous monitoring of cell growth, which may facilitate more accurate estimates of the growth states of lactic acid bacterial populations during fermented batch culture in industry. |
| 学科主题 | 工程技术 |
| 语种 | 英语 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9864]  |
| 专题 | 青岛生物能源与过程研究所_单细胞中心 |
| 通讯作者 | Heping Zhang |
| 推荐引用方式 GB/T 7714 | Yan Ren, Yuetong Ji, Lin Teng, Heping Zhang,Heping Zhang. Using Raman spectroscopy and chemometrics to identify the growth phase of Lactobacillus casei Zhang during batch culture at the single-cell level.[J]. Microbial Cell Factories,2017,16(1):233-243. |
| APA | Yan Ren, Yuetong Ji, Lin Teng, Heping Zhang,&Heping Zhang.(2017).Using Raman spectroscopy and chemometrics to identify the growth phase of Lactobacillus casei Zhang during batch culture at the single-cell level..Microbial Cell Factories,16(1),233-243. |
| MLA | Yan Ren, Yuetong Ji, Lin Teng, Heping Zhang,et al."Using Raman spectroscopy and chemometrics to identify the growth phase of Lactobacillus casei Zhang during batch culture at the single-cell level.".Microbial Cell Factories 16.1(2017):233-243. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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