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Producing Designer Oils in Industrial Microalgae by Rational Modulation of Co-evolving Type-2 Diacylglycerol Acyltransferases

文献类型：期刊论文

作者Xin, Yi1,2,10; Lu, Yandu1,2,10; Lee, Yi-Ying3,4; Wei, Li1,2,10; Jia, Jing1,2,10; Wang, Qintao1,2,10; Wang, Dongmei1,2,10; Bai, Fali9,10; Hu, Hanhua8,10; Hu, Qiang7,10
刊名MOLECULAR PLANT
出版日期2017-12-04
卷号10期号:12页码:1523-1539
关键词Biofuels Degree Of Unsaturation Genetic Engineering Diacylglycerol Acyltransferase Nannochloropsis
DOI10.1016/j.molp.2017.10.011
文献子类Article
英文摘要Microalgal oils, depending on their degree of unsaturation, can be utilized as either nutritional supplements or fuels; thus, a feedstock with genetically designed and tunable degree of unsaturation is desirable to maximize process efficiency and product versatility. Systematic profiling of ex vivo (in yeast), in vitro, and in vivo activities of type-2 diacylglycerol acyltransferases in Nannochloropsis oceanica (NoDGAT2s or NoDGTTs), via reverse genetics, revealed that NoDGAT2A prefers saturated fatty acids (SFAs), NoDGAT2D prefers monounsaturated fatty acids (MUFAs), and NoDGAT2C exhibits the strongest activity toward polyunsaturated fatty acids (PUFAs). As NoDGAT2A, 2C, and 2D originated from the green alga, red alga, and eukaryotic host ancestral participants of secondary endosymbiosis, respectively, a mechanistic model of oleaginousness was unveiled, in which the indigenous and adopted NoDGAT2s formulated functional complementarity and specific transcript abundance ratio that underlie a rigid SFA: MUFA:PUFA hierarchy in triacylglycerol (TAG). By rationally modulating the ratio of NoDGAT2A:2C:2D transcripts, a bank of N. oceanica strains optimized for nutritional supplement or fuel production with a wide range of degree of unsaturation were created, in which proportion of SFAs, MUFAs, and PUFAs in TAG varied by 1.3-, 3.7-, and 11.2-fold, respectively. This established a novel strategy to simultaneously improve productivity and quality of oils from industrial microalgae.
WOS关键词FATTY-ACID-COMPOSITION ; NANNOCHLOROPSIS-OCEANICA ; TRIACYLGLYCEROL BIOSYNTHESIS ; OLEAGINOUS MICROALGA ; EICOSAPENTAENOIC ACID ; ENDOPLASMIC-RETICULUM ; SYNTHETIC BIOLOGY ; GENE ; IDENTIFICATION ; CHLAMYDOMONAS
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
项目编号(31425002, 31600059, 31571807, 31401116, ZR2015CQ003
语种英语
WOS记录号WOS:000417204100005
资助机构Natural Science Foundation of China(31425002 ; Chinese Academy of Sciences(KSZD-EW-Z-017 ; Natural Science Foundation of Shandong(ZR2015CQ003) ; U.S. National Science Foundation(CBET-1511939) ; U.S. Office of Naval Research(N00014-15-1-2219) ; 31600059 ; ZDRW-ZS-2016-3) ; 31571807 ; 31401116)
源URL[http://ir.qibebt.ac.cn/handle/337004/9865]  
专题青岛生物能源与过程研究所_单细胞中心
通讯作者Xu J(徐健)
作者单位1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
3.Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21201 USA
4.Univ Maryland Baltimore Cty, Baltimore, MD 21202 USA
5.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
6.Peking Univ, Coll Engn, Dept Energy & Resource Engn, Beijing 100871, Peoples R China
7.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China
8.Chinese Acad Sci, Inst Hydrobiol, Diatom Biol Grp, Wuhan 430072, Hubei, Peoples R China
9.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Core Lab, Qingdao 266101, Shandong, Peoples R China
10.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Xin, Yi,Lu, Yandu,Lee, Yi-Ying,et al. Producing Designer Oils in Industrial Microalgae by Rational Modulation of Co-evolving Type-2 Diacylglycerol Acyltransferases[J]. MOLECULAR PLANT,2017,10(12):1523-1539.
APA Xin, Yi.,Lu, Yandu.,Lee, Yi-Ying.,Wei, Li.,Jia, Jing.,...&徐健.(2017).Producing Designer Oils in Industrial Microalgae by Rational Modulation of Co-evolving Type-2 Diacylglycerol Acyltransferases.MOLECULAR PLANT,10(12),1523-1539.
MLA Xin, Yi,et al."Producing Designer Oils in Industrial Microalgae by Rational Modulation of Co-evolving Type-2 Diacylglycerol Acyltransferases".MOLECULAR PLANT 10.12(2017):1523-1539.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

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