Enhanced poly(3-hydroxypropionate) production via β-alanine pathway in recombinant Escherichia coli.
文献类型：期刊论文
| 作者 | Stephen Tamekou Lacmata, Jules-Roger Kuiate, Yamei Ding, Mo Xian, Huizhou Liu, Thadde´e Boudjeko, Xinjun Feng, Guang Zhao.; Xinjun Feng ; Guang Zhao.
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| 刊名 | PLoS ONE
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| 出版日期 | 2017-03-02 |
| 卷号 | 12期号:3页码:1-11 |
| DOI | 10.1371/journal.pone.0173150 |
| 文献子类 | Article |
| 英文摘要 | Poly(3-hydroxypropionate) (P3HP) is a thermoplastic with great compostability and biocompatibility, and can be produced through several biosynthetic pathways, in which the glycerol pathway achieved the highest P3HP production. However, exogenous supply of vitamin B12 was required to maintain the activity of glycerol dehydratase, resulting in high production cost. To avoid the addition of VB12, we have previously constructed a P3HP biosynthetic route with β-alanine as intermediate, and the present study aimed to improve the P3HP production of this pathway. L-aspartate decarboxylase PanD was found to be the rate-limiting enzyme in the β-alanine pathway firstly. To improve the pathway efficiency, PanD was screened from four different sources (Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens, and Corynebacterium glutamicum). And PanD from C. glutamicum was found to have the highest activity, the P3HP production was improved in flask cultivation with this enzyme. To further improve the production, the host strain was screened and the culture condition was optimized. Under optimal conditions, production and content of P3HP reached to 10.2 g/L and 39.1% (wt/wt [cell dry weight]) in an aerobic fed-batch fermentation. To date, this is the highest P3HP production without VB12 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9908]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 通讯作者 | Xinjun Feng; Guang Zhao. |
| 作者单位 | 中国科学院青岛生物能源与过程研究所(2)喀麦隆杜昌大学(3)中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Stephen Tamekou Lacmata, Jules-Roger Kuiate, Yamei Ding, Mo Xian, Huizhou Liu, Thadde´e Boudjeko, Xinjun Feng, Guang Zhao.,Xinjun Feng,Guang Zhao.. Enhanced poly(3-hydroxypropionate) production via β-alanine pathway in recombinant Escherichia coli.[J]. PLoS ONE,2017,12(3):1-11. |
| APA | Stephen Tamekou Lacmata, Jules-Roger Kuiate, Yamei Ding, Mo Xian, Huizhou Liu, Thadde´e Boudjeko, Xinjun Feng, Guang Zhao.,Xinjun Feng,&Guang Zhao..(2017).Enhanced poly(3-hydroxypropionate) production via β-alanine pathway in recombinant Escherichia coli..PLoS ONE,12(3),1-11. |
| MLA | Stephen Tamekou Lacmata, Jules-Roger Kuiate, Yamei Ding, Mo Xian, Huizhou Liu, Thadde´e Boudjeko, Xinjun Feng, Guang Zhao.,et al."Enhanced poly(3-hydroxypropionate) production via β-alanine pathway in recombinant Escherichia coli.".PLoS ONE 12.3(2017):1-11. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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