Enzymatic process optimization for the in vitro production of isoprene from mevalonate.
文献类型：期刊论文
| 作者 | Tao Cheng; Hui Liu; Huibin Zou; Ningning Chen; Mengxun Shi; Congxia Xie; , Guang Zhao; and Mo Xian; Guang Zhao; Mo Xian. |
| 刊名 | Microbial Cell Factories
 |
| 出版日期 | 2017 |
| 卷号 | 16页码:8-16 |
| 关键词 | Mevalonate Isoprene Synthetic Biochemistry Isoprenoids Bio-based Chemicals Enzymatic Process |
| DOI | 10.1186/s12934-016-0622-4 |
| 文献子类 | Article |
| 英文摘要 | Background: As an important bulk chemical for synthetic rubber, isoprene can be biosynthesized by robust microbes. But rational engineering and optimization are often demanded to make the in vivo process feasible due to the complexities of cellular metabolism. Alternative synthetic biochemistry strategies are in fast development to produce isoprene or isoprenoids in vitro. Results: This study set up an in vitro enzyme synthetic chemistry process using 5 enzymes in the lower mevalonate pathway to produce isoprene from mevalonate. We found the level and ratio of individual enzymes would signifcantly affect the efciency of the whole system. The optimized process using 10 balanced enzyme unites (5.0 µM of MVK, PMK, MVD; 10.0 µM of IDI, 80.0 µM of ISPS) could produce 6323.5 µmol/L/h (430 mg/L/h) isoprene in a 2 ml in vitro system. In a scale up process (50 ml) only using 1 balanced enzyme unit (0.5 µM of MVK, PMK, MVD; 1.0 µM of IDI, 8.0 µM of ISPS), the system could produce 302 mg/L isoprene in 40 h, which showed higher production rate and longer reaction phase with comparison of the in vivo control. Conclusions: By optimizing the enzyme levels of lower MVA pathway, synthetic biochemistry methods could be set up for the enzymatic production of isoprene or isoprenoids from mevalonate. |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9909]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 通讯作者 | Huibin Zou; Guang Zhao; Mo Xian. |
| 作者单位 | 中国科学院青岛生物能源与过程研究所(2)青岛科技大学 |
| 推荐引用方式 GB/T 7714 | Tao Cheng,Hui Liu,Huibin Zou,et al. Enzymatic process optimization for the in vitro production of isoprene from mevalonate.[J]. Microbial Cell Factories,2017,16:8-16. |
| APA | Tao Cheng.,Hui Liu.,Huibin Zou.,Ningning Chen.,Mengxun Shi.,...&Mo Xian..(2017).Enzymatic process optimization for the in vitro production of isoprene from mevalonate..Microbial Cell Factories,16,8-16. |
| MLA | Tao Cheng,et al."Enzymatic process optimization for the in vitro production of isoprene from mevalonate.".Microbial Cell Factories 16(2017):8-16. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。