microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri
文献类型：期刊论文
| 作者 | Liao, X; Yang, L; Zhang, QL; Chen, JY (陈均远)
|
| 刊名 | Developmental and Comparative Immunology
 |
| 出版日期 | 2017 |
| 卷号 | 70页码:39-44 |
| 关键词 | microRNA Innate immune Branchiostoma belcheri Lipopolysaccharide |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2017.01.007 |
| 英文摘要 | Recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus. In this article, we identified the amphioxus miRNAs in the acute-phase response to lipopolysaccharide (LPS). We determined the time point for the peak of immune response in amphioxus after LPS challenge by evaluating the expression of Branchiostoma belcheri toll-like receptor 1, NF-icb (c-Rel), and big defensin which react with pathogen-associated molecular pattems(PAMPs). Then we chose 12 h as the point to perform miRNA microarray analysis to select the differentially expressed miRNAs. Furthermore, we used quantitative real-time PCR to detect the expression patterns of selected amphioxus miRNAs under effective LPS challenge during the time course. The microarray data revealed that the miRNA expression file was significantly changed after LPS stimulation. The changes of the 10 most upregulated and 7 most downregualted miRNAs in gills of the amphioxus following challenge with LPS revealed a temporal induction kinetic. Our current study will provide valuable information to take an insight into molecular mechanism of innate immune and the evolution of the miRNA family. (C) 2017 Elsevier Ltd. All rights reserved. |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000394635500005 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.nigpas.ac.cn/handle/332004/13653]  |
| 专题 | 中国科学院南京地质古生物研究所 南京地质古生物研究所_其他 |
| 通讯作者 | Chen, JY (陈均远) |
| 推荐引用方式 GB/T 7714 | Liao, X,Yang, L,Zhang, QL,et al. microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri[J]. Developmental and Comparative Immunology,2017,70:39-44. |
| APA | Liao, X,Yang, L,Zhang, QL,&Chen, JY .(2017).microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri.Developmental and Comparative Immunology,70,39-44. |
| MLA | Liao, X,et al."microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri".Developmental and Comparative Immunology 70(2017):39-44. |
入库方式： OAI收割
来源：南京地质古生物研究所
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