Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death
文献类型：期刊论文
| 作者 | Wu-Zhen Liu; Fangfang Niu; Jingxiao Zhang; Bo Yang; Yuan-Qing Jiang; Bisi Chen; Hao Cheng; Jieyu Ma; Feng Han |
| 刊名 | DNA Research
 |
| 出版日期 | 2016 |
| 卷号 | 23期号:2页码:101-14 |
| 关键词 | Abiotic Stress Brassica Napus Myb Ros Cell Death |
| DOI | 10.1093/dnares/dsv040 |
| 文献子类 | Article |
| 英文摘要 | The R2R3-MYB proteins comprise one of the largest families of transcription factors in plants. Although genome-wide analysis of this family has been carried out in some plant species, little is known about R2R3-MYB genes in canola (Brassica napus L.). In this study, we have identified 76 R2R3-MYB genes in the canola genome through mining of expressed sequence tags (ESTs). The cDNA sequences of 44 MYB genes were successfully cloned. The transcriptional activities of BnaMYB proteins encoded by these genes were assayed in yeast. The subcellular localizations of representative R2R3-MYB proteins were investigated through GFP fusion. Besides, the transcript abundance level analysis during abiotic conditions and ABA treatment identified a group of R2R3- MYB genes that responded to one or more treatments. Furthermore, we identified a previously functionally unknown MYB gene-BnaMYB78, which modulates reactive oxygen species (ROS)- dependent cell death in Nicotiana benthamiana, through regulating the transcription of a few ROS- and defence-related genes. Taken together, this study has provided a solid foundation for understanding the roles and regulatory mechanism of canola R2R3-MYB genes. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000375088800003 |
| 源URL | [http://ir.iswc.ac.cn/handle/361005/7815]  |
| 专题 | 水土保持研究所_水保所科研产出--SCI_2016--SCI |
| 通讯作者 | Yuan-Qing Jiang |
| 作者单位 | State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China |
| 推荐引用方式 GB/T 7714 | Wu-Zhen Liu,Fangfang Niu,Jingxiao Zhang,et al. Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death[J]. DNA Research,2016,23(2):101-14. |
| APA | Wu-Zhen Liu.,Fangfang Niu.,Jingxiao Zhang.,Bo Yang.,Yuan-Qing Jiang.,...&Feng Han.(2016).Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death.DNA Research,23(2),101-14. |
| MLA | Wu-Zhen Liu,et al."Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death".DNA Research 23.2(2016):101-14. |
入库方式： OAI收割
来源：水土保持研究所
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