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A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L

文献类型：期刊论文

作者Peng, Liang4; Liang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310000, Zhejiang, Peoples R China.; Ru, Mei2,3; Wang, Kunru2,3; Bai, Zhenqing2,3; He, Shaoxuan1; Wang, Yong2,3; Liang, Zongsuo2,3; Liang, ZS (reprint author), Minist Water Resources, Yangling 712100, Peoples R China.
刊名SCIENTIFIC REPORTS
出版日期2017
卷号7
ISSN号2045-2322
DOI10.1038/s41598-017-05290-4
文献子类Article
英文摘要Rosmarinic acid (RA) and its derivants are medicinal compounds that comprise the active components of several therapeutics. We isolated and characterised a tyrosine aminotransferase of Prunella vulgaris (PvTAT). Deduced PvTAT was markedly homologous to other known/putative plant TATs. Cytoplasmic localisation of PvTAT was observed in tobacco protoplasts. Recombinantly expressed and purified PvTAT had substrates preference for L-tyrosine and phenylpyruvate, with apparent K-m of 0.40 and 0.48 mM, and favoured the conversion of tyrosine to 4-hydroxyphenylpyruvate. In vivo activity was confirmed by functional restoration of the Escherichia coli tyrosine auxotrophic mutant DL39. Agrobacterium rhizogenes-mediated antisense/sense expression of PvTAT in hairy roots was used to evaluate the contribution of PvTAT to RA synthesis. PvTAT were reduced by 46-95% and RA were decreased by 36-91% with low catalytic activity in antisense transgenic hairy root lines; furthermore, PvTAT were increased 0.77-2.6- fold with increased 1.3-1.8-fold RA and strong catalytic activity in sense transgenic hairy root lines compared with wild-type counterparts. The comprehensive physiological and catalytic evidence fills in the gap in RA-producing plants which didn't provide evidence for TAT expression and catalytic activities in vitro and in vivo. That also highlights RA biosynthesis pathway in P. vulgaris and provides useful information to engineer natural products.
学科主题Science & Technology - Other Topics
URL标识查看原文
出版地LONDON
语种英语
WOS记录号WOS:000404970900054
出版者NATURE PUBLISHING GROUP
资助机构Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; National Natural Science Foundation of China [81373908] ; Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; National Natural Science Foundation of China [81373908] ; Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; National Natural Science Foundation of China [81373908] ; Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; National Natural Science Foundation of China [81373908]
源URL[http://ir.iswc.ac.cn/handle/361005/7961]  
专题水保所科研产出--SCI_2017--SCI
通讯作者Liang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310000, Zhejiang, Peoples R China.; Liang, ZS (reprint author), Minist Water Resources, Yangling 712100, Peoples R China.
作者单位1.Environm Protect Agcy, Ecol Environm Monitoring Stn, Dazu 402360, Peoples R China
2.Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
3.Minist Water Resources, Yangling 712100, Peoples R China
4.Shannxi Univ Chinese Med, Coll Pharm, Xian 710000, Peoples R China
5.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310000, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Peng, Liang,Liang, ZS ,Liang, ZS ,et al. A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L[J]. SCIENTIFIC REPORTS,2017,7.
APA Peng, Liang.,Liang, ZS .,Liang, ZS .,Ru, Mei.,Wang, Kunru.,...&Liang, ZS .(2017).A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L.SCIENTIFIC REPORTS,7.
MLA Peng, Liang,et al."A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L".SCIENTIFIC REPORTS 7(2017).

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来源：水土保持研究所

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