Enhanced 2-keto-L-gulonic acid production by applying L-sorbose-tolerant helper strain in the co-culture system
文献类型：期刊论文
| 作者 | Mandlaa1,3; Sun, Ziyu1; Wang, Ruigang2; Han, Xiaodong2; Xu, Hui3; Yang, Weichao3 |
| 刊名 | AMB EXPRESS
 |
| 出版日期 | 2018-02-28 |
| 卷号 | 8 |
| 关键词 | 2-keto-gulonic Acid Co-culture System Helper Strain L-sorbose Vitamin c |
| ISSN号 | 2191-0855 |
| DOI | 10.1186/s13568-018-0562-y |
| 英文摘要 | 2-Keto-L-gulonic acid (the precursor of vitamin C) is bio-converted from L-sorbose by mixed fermentation of Ketogulo-nicigenium vulgare and a helper strain. The helper strain promotes the conversion of 2-KLG by enhancing the growth of K. vulgare, but its growth is greatly inhibited by high concentration of L-sorbose, which consequently influence the 2-KLG production. The aim of this study is to obtain L-sorbose-tolerant helper strain (LHS) by experimental evolution for reduced L-sorbose-inhibition-effect and enhanced 2-KLG productivity in high concentration of l-sorbose. After three steps screening by using our devised screening strategy, three strains (i.e., Bc 21, Bc 47, Bc 50) with high resistance to high concentration of L-sorbose were obtained. The fermentation tests by co-culturing Bc 21 and K. vulgare 418 showed that the production of 2-KLG was increased by 17.9% in 11% L-sorbose medium than that in 8% after 55 h of fermentation and the conversion rate was 89.5%. The results suggested that Bc 21 could be an ideal helper strain for 2-KLG production under high concentration of L-sorbose and demonstrated the feasibility of using experimental evolution to breed LHS for vitamin C production. |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000426692400001 |
| 出版者 | BIOMED CENTRAL LTD |
| 源URL | [http://210.72.129.5/handle/321005/109262]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 通讯作者 | Yang, Weichao |
| 作者单位 | 1.Inner Mongolia Agr Univ, Coll Food Sci & Engn, Hohhot 010018, Peoples R China 2.Inner Mongolia Agr Univ, Coll Life Sci, Hohhot 010018, Peoples R China 3.Chinese Acad Sci, Inst Appl Ecol, 72 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mandlaa,Sun, Ziyu,Wang, Ruigang,et al. Enhanced 2-keto-L-gulonic acid production by applying L-sorbose-tolerant helper strain in the co-culture system[J]. AMB EXPRESS,2018,8. |
| APA | Mandlaa,Sun, Ziyu,Wang, Ruigang,Han, Xiaodong,Xu, Hui,&Yang, Weichao.(2018).Enhanced 2-keto-L-gulonic acid production by applying L-sorbose-tolerant helper strain in the co-culture system.AMB EXPRESS,8. |
| MLA | Mandlaa,et al."Enhanced 2-keto-L-gulonic acid production by applying L-sorbose-tolerant helper strain in the co-culture system".AMB EXPRESS 8(2018). |
入库方式： OAI收割
来源：沈阳应用生态研究所
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