method for preparing a sterile transgenic carp
文献类型：专利
| 作者 | Hu W(胡炜) ; Zhu ZY(朱作言) ; Wang YP(汪亚平) ; Li SF(黎双飞) |
| 发表日期 | 2007-06-12 |
| 专利国别 | 美国 |
| 专利号 | US7230156B2 |
| 专利类型 | 发明 |
| 权利人 | 中国科学院水生生物研究所 |
| 中文摘要 | The present invention discloses a preparation method of sterile transgenic fish, and said method includes the following steps: using carp actin gene promoter as promotor, using antisense 296 bp carp salmon type gonadotropin-releasing hormone gene complementary DNA fragment [including sGnRH+peptide and gonadotropin-releasing hormone related peptide coding zone and 3' non-coding zone sequence of 64 bp] as target gene, and using grass carp growth hormone gene 3' flanking sequence as termination sequence to construct salmon type gonadotropin-releasing hormone antisense RNA expression vector, and adopting microinjection method to introduce the recombinant DNA fragment into carp fertilized egg, and utilizing PCR reaction and radioimmunodetection to screen and obtain the invented sterile transgenic fish. |
| 公开日期 | 2010-11-03 |
| 申请日期 | 2005-03-31 |
| 语种 | 英语 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/11494]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_专利 |
| 推荐引用方式 GB/T 7714 | Hu W,Zhu ZY,Wang YP,et al. method for preparing a sterile transgenic carp. US7230156B2. 2007-06-12. |
入库方式： OAI收割
来源：水生生物研究所
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