中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
method for preparing a sterile transgenic carp

文献类型:专利

作者Hu W(胡炜) ; Zhu ZY(朱作言) ; Wang YP(汪亚平) ; Li SF(黎双飞)
发表日期2007-06-12
专利国别美国
专利号US7230156B2
专利类型发明
权利人中国科学院水生生物研究所
中文摘要The present invention discloses a preparation method of sterile transgenic fish, and said method includes the following steps: using carp actin gene promoter as promotor, using antisense 296 bp carp salmon type gonadotropin-releasing hormone gene complementary DNA fragment [including sGnRH+peptide and gonadotropin-releasing hormone related peptide coding zone and 3' non-coding zone sequence of 64 bp] as target gene, and using grass carp growth hormone gene 3' flanking sequence as termination sequence to construct salmon type gonadotropin-releasing hormone antisense RNA expression vector, and adopting microinjection method to introduce the recombinant DNA fragment into carp fertilized egg, and utilizing PCR reaction and radioimmunodetection to screen and obtain the invented sterile transgenic fish.
公开日期2010-11-03
申请日期2005-03-31
语种英语
源URL[http://ir.ihb.ac.cn/handle/342005/11494]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_专利
推荐引用方式
GB/T 7714
Hu W,Zhu ZY,Wang YP,et al. method for preparing a sterile transgenic carp. US7230156B2. 2007-06-12.

入库方式: OAI收割

来源:水生生物研究所

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