Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis
文献类型：期刊论文
| 作者 | Li, Xuemei; Yu, Yuhe; Xie, Shouqi; Yan, Qingyun; Chen, Yuhang; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China |
| 刊名 | JOURNAL OF THE WORLD AQUACULTURE SOCIETY
 |
| 出版日期 | 2011 |
| 卷号 | 42期号:4页码:539-548 |
| 关键词 | Trout Oncorhynchus-mykiss 16s Ribosomal-rna Early-life Stages Sole Solea-Solea Fresh-water Fish Phylogenetic Analysis Molecular-identification Gastrointestinal-tract Microbial Community Cyprinus-carpio |
| ISSN号 | 0893-8849 |
| 英文摘要 | A 16S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method was applied to detect intestinal bacterial communities of juvenile allogynogenetic crucian carp, Carassius auratus gibelio, fed with chitosan-containing diet. This is the first time to use the molecular method to analyze the bacterial communities in the allogynogenetic crucian carp intestine. The DGGE profile with universal bacterial primers revealed simple communities in all treatment groups. Sequencing and phylogenetic analysis of excised DGGE bands showed that the dominant bacteria belonged to class gamma-Proteobacteria and Fusobacteria. The relative abundance and diversity of detected bacteria suggested that 0.5 and 0.75% of chitosan in diet were optimum for juvenile allogynogenetic crucian carp. As in these concentrations, some detected pathogen bacteria either disappeared or decreased. However, the DGGE profile with Aeromonas-specific primers showed a similar composition among all treatment groups, which suggested that Aeromonas was one of relative stable bacteria components in the intestine of juvenile allogynogenetic crucian carp. |
| 学科主题 | Fisheries |
| 语种 | 英语 |
| WOS记录号 | WOS:000293351200008 |
| 资助机构 | Major State Basic Research Development Program of China[2009CB118705, 2007CB109205] ; Major State Basic Research Development Program of China[2009CB118705, 2007CB109205] ; Major State Basic Research Development Program of China[2009CB118705, 2007CB109205] ; Major State Basic Research Development Program of China[2009CB118705, 2007CB109205] |
| 公开日期 | 2011-11-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16281]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 通讯作者 | Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xuemei,Yu, Yuhe,Xie, Shouqi,et al. Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2011,42(4):539-548. |
| APA | Li, Xuemei,Yu, Yuhe,Xie, Shouqi,Yan, Qingyun,Chen, Yuhang,&Yu, YH .(2011).Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,42(4),539-548. |
| MLA | Li, Xuemei,et al."Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp, Carassius auratus gibelio, as Depicted by polymerase chain reaction-denaturing Gradient Gel Electrophoresis".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 42.4(2011):539-548. |
入库方式： OAI收割
来源：水生生物研究所
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