Temporal heterogeneity of plankton community in Lake Dianchi and its relation to environmental factors
文献类型：期刊论文
| 作者 | Wu, Li2; Feng, Weisong1; Feng, WS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China. |
| 刊名 | JOURNAL OF FRESHWATER ECOLOGY
 |
| 出版日期 | 2012-06-01 |
| 卷号 | 27期号:2页码:229-241 |
| 关键词 | Fingerprinting Community Structure Zooplankton Rapd Pcr-dgge Lake Dianchi |
| ISSN号 | 0270-5060 |
| DOI | 10.1080/02705060.2012.657828 |
| 文献子类 | Article |
| 英文摘要 | The plankton community in Fubao Bay of Lake Dianchi, a large, shallow and highly polluted freshwater lake in China, was investigated on three dates. First, a total of 76 zooplankton species were identified by morphological characteristics of which 44 were protozoa, 20 rotifer, and 12 crustacean. Then, the plankton community was characterized by using random amplified polymorphic DNA (RAPD) and Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) over the time period. Morphological composition, RAPD fingerprints, PCR-DGGE fingerprints, and unweighted pair-group method using arithmetic averages clustering analysis all showed that samples within a season were generally clustered into the same group. The results also revealed that RAPD fingerprints and PCR-DGGE fingerprints were identical to morphological composition in characterizing community similarity. Canonical correspondence analysis based on morphological composition, RAPD fingerprints, and PCR-DGGE fingerprints all suggested that the factors strongly correlated with the first two ordination axes were seasonally different, further suggesting high- temporal heterogeneity in Lake Dianchi. There were close relationships between morphological composition, RAPD and PCR-DGGE fingerprints, and: (1) total nitrogen in summer, (2) total phosphorus in autumn, and (3) BOD5 or NO2-N in winter. Results of this study suggest that molecular techniques are acceptable methods for examining plankton community composition and measuring plankton community responses to stress and perturbation.; The plankton community in Fubao Bay of Lake Dianchi, a large, shallow and highly polluted freshwater lake in China, was investigated on three dates. First, a total of 76 zooplankton species were identified by morphological characteristics of which 44 were protozoa, 20 rotifer, and 12 crustacean. Then, the plankton community was characterized by using random amplified polymorphic DNA (RAPD) and Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) over the time period. Morphological composition, RAPD fingerprints, PCR-DGGE fingerprints, and unweighted pair-group method using arithmetic averages clustering analysis all showed that samples within a season were generally clustered into the same group. The results also revealed that RAPD fingerprints and PCR-DGGE fingerprints were identical to morphological composition in characterizing community similarity. Canonical correspondence analysis based on morphological composition, RAPD fingerprints, and PCR-DGGE fingerprints all suggested that the factors strongly correlated with the first two ordination axes were seasonally different, further suggesting high- temporal heterogeneity in Lake Dianchi. There were close relationships between morphological composition, RAPD and PCR-DGGE fingerprints, and: (1) total nitrogen in summer, (2) total phosphorus in autumn, and (3) BOD5 or NO2-N in winter. Results of this study suggest that molecular techniques are acceptable methods for examining plankton community composition and measuring plankton community responses to stress and perturbation. |
| WOS关键词 | GRADIENT GEL-ELECTROPHORESIS ; GENETIC DIVERSITY ; TROPHIC STATUS ; WATER-QUALITY ; ECOSYSTEMS ; MICROORGANISMS ; POPULATIONS ; INDICATORS ; ECOLOGY ; DONGHU |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000304454400006 |
| 资助机构 | Major State Basic Research Development Program of China[2008CB418105]; Anhui Colleges[KJ2011Z308] ; Major State Basic Research Development Program of China[2008CB418105]; Anhui Colleges[KJ2011Z308] ; Major State Basic Research Development Program of China[2008CB418105]; Anhui Colleges[KJ2011Z308] ; Major State Basic Research Development Program of China[2008CB418105]; Anhui Colleges[KJ2011Z308] |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16855]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 通讯作者 | Feng, WS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China 2.Hefei Normal Univ, Dept Life Sci, Hefei 230061, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Li,Feng, Weisong,Feng, WS . Temporal heterogeneity of plankton community in Lake Dianchi and its relation to environmental factors[J]. JOURNAL OF FRESHWATER ECOLOGY,2012,27(2):229-241. |
| APA | Wu, Li,Feng, Weisong,&Feng, WS .(2012).Temporal heterogeneity of plankton community in Lake Dianchi and its relation to environmental factors.JOURNAL OF FRESHWATER ECOLOGY,27(2),229-241. |
| MLA | Wu, Li,et al."Temporal heterogeneity of plankton community in Lake Dianchi and its relation to environmental factors".JOURNAL OF FRESHWATER ECOLOGY 27.2(2012):229-241. |
入库方式： OAI收割
来源：水生生物研究所
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