中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Identification of cda gene in bighead carp and its expression in response to microcystin-LR

文献类型:期刊论文

作者Cai, Yan; Li, Huiying; Hao, Le; Li, Guangyu; Xie, Ping; Chen, Jun; Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
刊名ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
出版日期2012-05-01
卷号79期号:-页码:206-213
关键词Cda Gene Identification Mrna Expression Microcystin-lr Bighead Carp
ISSN号0147-6513
DOI10.1016/j.ecoenv.2012.01.001
文献子类Article
英文摘要Microcystin-LR (MCLR) is a widespread cyanotoxin, which can influence genes transcription and cause nucleic acid damage in different organisms. To identify MCLR induced transcriptionally changed hepatic genes in bighead carp by subtractive suppression hybridization, we obtained the cDNA fragment of cda. Then we cloned its full-length cDNA, which encodes a cytidine deaminase (CDA). 3D structure prediction showed that the 3D structure and amino acid residues related to function sites of bighead carp CDA were highly conserved. Bighead carp CDA shared high identities with other CDA sequences, and evolved closely to non-mammalian CDAs. Bighead carp expressed cda in all tested tissues under normal situation, and changed its expression profile in a time inversely dependent and dose dependent manner to MCLR, so as to protect itself from MCLR induced toxic damage. These indicated that cda might be involved in anti-MCLR response, especially in the regulation of cytidine and dexocytidine metabolism pathway. (C) 2012 Elsevier Inc. All rights reserved.; Microcystin-LR (MCLR) is a widespread cyanotoxin, which can influence genes transcription and cause nucleic acid damage in different organisms. To identify MCLR induced transcriptionally changed hepatic genes in bighead carp by subtractive suppression hybridization, we obtained the cDNA fragment of cda. Then we cloned its full-length cDNA, which encodes a cytidine deaminase (CDA). 3D structure prediction showed that the 3D structure and amino acid residues related to function sites of bighead carp CDA were highly conserved. Bighead carp CDA shared high identities with other CDA sequences, and evolved closely to non-mammalian CDAs. Bighead carp expressed cda in all tested tissues under normal situation, and changed its expression profile in a time inversely dependent and dose dependent manner to MCLR, so as to protect itself from MCLR induced toxic damage. These indicated that cda might be involved in anti-MCLR response, especially in the regulation of cytidine and dexocytidine metabolism pathway. (C) 2012 Elsevier Inc. All rights reserved.
WOS关键词HUMAN CYTIDINE DEAMINASE ; CRYSTAL-STRUCTURE ; IN-VIVO ; ARABIDOPSIS-THALIANA ; CYTOSINE-ARABINOSIDE ; CDNA SEQUENCES ; DNA-DAMAGE ; PURIFICATION ; EVOLUTION ; ENZYME
WOS研究方向Environmental Sciences & Ecology ; Toxicology
语种英语
WOS记录号WOS:000302107000029
资助机构National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
公开日期2012-09-25
源URL[http://ir.ihb.ac.cn/handle/342005/16877]  
专题水生生物研究所_淡水生态学研究中心_期刊论文
通讯作者Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
作者单位Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714
Cai, Yan,Li, Huiying,Hao, Le,et al. Identification of cda gene in bighead carp and its expression in response to microcystin-LR[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2012,79(-):206-213.
APA Cai, Yan.,Li, Huiying.,Hao, Le.,Li, Guangyu.,Xie, Ping.,...&Chen, J .(2012).Identification of cda gene in bighead carp and its expression in response to microcystin-LR.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,79(-),206-213.
MLA Cai, Yan,et al."Identification of cda gene in bighead carp and its expression in response to microcystin-LR".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 79.-(2012):206-213.

入库方式: OAI收割

来源:水生生物研究所

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