Identification of cda gene in bighead carp and its expression in response to microcystin-LR
文献类型：期刊论文
| 作者 | Cai, Yan; Li, Huiying; Hao, Le; Li, Guangyu; Xie, Ping; Chen, Jun; Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China. |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 79期号:-页码:206-213 |
| 关键词 | Cda Gene Identification Mrna Expression Microcystin-lr Bighead Carp |
| ISSN号 | 0147-6513 |
| DOI | 10.1016/j.ecoenv.2012.01.001 |
| 文献子类 | Article |
| 英文摘要 | Microcystin-LR (MCLR) is a widespread cyanotoxin, which can influence genes transcription and cause nucleic acid damage in different organisms. To identify MCLR induced transcriptionally changed hepatic genes in bighead carp by subtractive suppression hybridization, we obtained the cDNA fragment of cda. Then we cloned its full-length cDNA, which encodes a cytidine deaminase (CDA). 3D structure prediction showed that the 3D structure and amino acid residues related to function sites of bighead carp CDA were highly conserved. Bighead carp CDA shared high identities with other CDA sequences, and evolved closely to non-mammalian CDAs. Bighead carp expressed cda in all tested tissues under normal situation, and changed its expression profile in a time inversely dependent and dose dependent manner to MCLR, so as to protect itself from MCLR induced toxic damage. These indicated that cda might be involved in anti-MCLR response, especially in the regulation of cytidine and dexocytidine metabolism pathway. (C) 2012 Elsevier Inc. All rights reserved.; Microcystin-LR (MCLR) is a widespread cyanotoxin, which can influence genes transcription and cause nucleic acid damage in different organisms. To identify MCLR induced transcriptionally changed hepatic genes in bighead carp by subtractive suppression hybridization, we obtained the cDNA fragment of cda. Then we cloned its full-length cDNA, which encodes a cytidine deaminase (CDA). 3D structure prediction showed that the 3D structure and amino acid residues related to function sites of bighead carp CDA were highly conserved. Bighead carp CDA shared high identities with other CDA sequences, and evolved closely to non-mammalian CDAs. Bighead carp expressed cda in all tested tissues under normal situation, and changed its expression profile in a time inversely dependent and dose dependent manner to MCLR, so as to protect itself from MCLR induced toxic damage. These indicated that cda might be involved in anti-MCLR response, especially in the regulation of cytidine and dexocytidine metabolism pathway. (C) 2012 Elsevier Inc. All rights reserved. |
| WOS关键词 | HUMAN CYTIDINE DEAMINASE ; CRYSTAL-STRUCTURE ; IN-VIVO ; ARABIDOPSIS-THALIANA ; CYTOSINE-ARABINOSIDE ; CDNA SEQUENCES ; DNA-DAMAGE ; PURIFICATION ; EVOLUTION ; ENZYME |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000302107000029 |
| 资助机构 | National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16877]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 通讯作者 | Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China. |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Yan,Li, Huiying,Hao, Le,et al. Identification of cda gene in bighead carp and its expression in response to microcystin-LR[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2012,79(-):206-213. |
| APA | Cai, Yan.,Li, Huiying.,Hao, Le.,Li, Guangyu.,Xie, Ping.,...&Chen, J .(2012).Identification of cda gene in bighead carp and its expression in response to microcystin-LR.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,79(-),206-213. |
| MLA | Cai, Yan,et al."Identification of cda gene in bighead carp and its expression in response to microcystin-LR".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 79.-(2012):206-213. |
入库方式： OAI收割
来源：水生生物研究所
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