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Identification of a pou2 ortholog in Chinese sturgeon, Acipenser sinensis and its expression patterns in tissues, immature individuals and during embryogenesis

文献类型：期刊论文

作者Ye, Huan1,2; Du, Hao1,3; Chen, Xi-Hua1; Cao, Hong4; Liu, Tao1,2; Li, Chuang-Ju1,3; Li, CJ (reprint author), Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China.
刊名FISH PHYSIOLOGY AND BIOCHEMISTRY
出版日期2012-08-01
卷号38期号:4页码:929-942
关键词Chinese Sturgeon Aspou2 Cloning Gene Expression Rt-pcr
ISSN号0920-1742
DOI10.1007/s10695-011-9579-8
文献子类Article
英文摘要The class V POU family genes, including pou5f1 and pou2, encode transcription factors critical for the maintenance of pluripotency in embryonic stem cells (ESC) and germ line cells in vertebrates. In the present study, the full-length cDNA of a pou2 ortholog in A. sinensis, Aspou2, was cloned and sequenced. This cDNA sequence is 2,853 base pairs in length and encodes a peptide of 431 amino acid residues. A comparison of the deduced amino acid sequence of Aspou2 with that of other vertebrate species showed that they were highly conserved in the POU domain, which shared 88 and 90% identity with that of zebrafish and medaka, respectively, and was 69, 67 and 67% identical to frog, mouse and human, respectively. RT-PCR analysis revealed that Aspou2 was detected in all tissues examined except for the liver, and high mRNA levels of Aspou2 were found in the muscle, pituitary and brain. During the embryogenesis and early larval development, the expression level of Aspou2 mRNAs decreased gradually apart from 1-day larvae that were not observed. Furthermore, Aspou2 seemed to raise with the development of gonads of immature Chinese sturgeons. These results suggested the possible involvement of Aspou2 in the nonpluripotent cells, pluripotent cells, embryogenesis, and gonad development.; The class V POU family genes, including pou5f1 and pou2, encode transcription factors critical for the maintenance of pluripotency in embryonic stem cells (ESC) and germ line cells in vertebrates. In the present study, the full-length cDNA of a pou2 ortholog in A. sinensis, Aspou2, was cloned and sequenced. This cDNA sequence is 2,853 base pairs in length and encodes a peptide of 431 amino acid residues. A comparison of the deduced amino acid sequence of Aspou2 with that of other vertebrate species showed that they were highly conserved in the POU domain, which shared 88 and 90% identity with that of zebrafish and medaka, respectively, and was 69, 67 and 67% identical to frog, mouse and human, respectively. RT-PCR analysis revealed that Aspou2 was detected in all tissues examined except for the liver, and high mRNA levels of Aspou2 were found in the muscle, pituitary and brain. During the embryogenesis and early larval development, the expression level of Aspou2 mRNAs decreased gradually apart from 1-day larvae that were not observed. Furthermore, Aspou2 seemed to raise with the development of gonads of immature Chinese sturgeons. These results suggested the possible involvement of Aspou2 in the nonpluripotent cells, pluripotent cells, embryogenesis, and gonad development.
WOS关键词HUMAN CHORIONIC-GONADOTROPIN ; TRANSCRIPTION FACTOR OCT-3/4 ; EMBRYONIC STEM-CELLS ; DEVELOPMENTAL EXPRESSION ; MOLECULAR CHARACTERIZATION ; DIFFERENTIAL EXPRESSION ; MAMMALIAN DEVELOPMENT ; BETA-SUBUNIT ; GERM-LINE ; OCT4
WOS研究方向Biochemistry & Molecular Biology ; Fisheries ; Physiology
语种英语
WOS记录号WOS:000305980200003
资助机构National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries[2011 JBFA22]; National Natural Science Foundation of China[31001104]; fund of Three-Gorges Project Corporation for Chinese sturgeon conservation[0799518]; fund of Central budget for running expense of Chinese sturgeon conservation ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries[2011 JBFA22]; National Natural Science Foundation of China[31001104]; fund of Three-Gorges Project Corporation for Chinese sturgeon conservation[0799518]; fund of Central budget for running expense of Chinese sturgeon conservation ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries[2011 JBFA22]; National Natural Science Foundation of China[31001104]; fund of Three-Gorges Project Corporation for Chinese sturgeon conservation[0799518]; fund of Central budget for running expense of Chinese sturgeon conservation ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center; Chinese Academy of Fisheries[2011 JBFA22]; National Natural Science Foundation of China[31001104]; fund of Three-Gorges Project Corporation for Chinese sturgeon conservation[0799518]; fund of Central budget for running expense of Chinese sturgeon conservation
公开日期2012-09-25
源URL[http://ir.ihb.ac.cn/handle/342005/17031]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者Li, CJ (reprint author), Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China.
作者单位1.Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714		 Ye, Huan,Du, Hao,Chen, Xi-Hua,et al. Identification of a pou2 ortholog in Chinese sturgeon, Acipenser sinensis and its expression patterns in tissues, immature individuals and during embryogenesis[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2012,38(4):929-942.
APA Ye, Huan.,Du, Hao.,Chen, Xi-Hua.,Cao, Hong.,Liu, Tao.,...&Li, CJ .(2012).Identification of a pou2 ortholog in Chinese sturgeon, Acipenser sinensis and its expression patterns in tissues, immature individuals and during embryogenesis.FISH PHYSIOLOGY AND BIOCHEMISTRY,38(4),929-942.
MLA Ye, Huan,et al."Identification of a pou2 ortholog in Chinese sturgeon, Acipenser sinensis and its expression patterns in tissues, immature individuals and during embryogenesis".FISH PHYSIOLOGY AND BIOCHEMISTRY 38.4(2012):929-942.

入库方式： OAI收割

来源：水生生物研究所

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