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Conformational changes in photosynthetic pigment proteins on thylakoid membranes can lead to fast non-photochemical quenching in cyanobacteria

文献类型：期刊论文

作者Wang ZhiCong1,2; Dong Jing1,2; Li DunHai1; Li, DH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
刊名SCIENCE CHINA-LIFE SCIENCES
出版日期2012-08-01
卷号55期号:8页码:726-734
关键词Chlorophyll Fluorescence Photoprotection Microcystis Aeruginosa Non-photochemical Quenching Thylakoid Membrane Proteins
ISSN号1674-7305
DOI10.1007/s11427-012-4360-5
文献子类Article
英文摘要A high non-photochemical quenching (NPQ) appeared below the phase transition temperature when Microcystis aeruginosa PCC7806 cells were exposed to saturated light for a short time. This suggested that a component of NPQ, independent from state transition or photo-inhibition, had been generated in the PSII complex; this was a fast component responding to high intensity light. Glutaraldehyde (GA), commonly used to stabilize membrane protein conformations, resulted in more energy transfer to PSII reaction centers, affecting the energy absorption and dissipation process rather than the transfer process of phycobilisome (PBS). In comparison experiments with and without GA, the rapid light curves (RLCs) and fluorescence induction dynamics of the fast phase showed that excess excitation energy was dissipated by conformational change in the photosynthetic pigment proteins on the thylakoid membrane (PPPTM). Based on deconvolution of NPQ relaxation kinetics, we concluded that the fast quenching component (NPQf) was closely related to PPPTM conformational change, as it accounted for as much as 39.42% of the total NPQ. We hypothesize therefore, that NPQf induced by PPPTM conformation is an important adaptation mechanism for Microcystis blooms under high-intensity light during summer and autumn.; A high non-photochemical quenching (NPQ) appeared below the phase transition temperature when Microcystis aeruginosa PCC7806 cells were exposed to saturated light for a short time. This suggested that a component of NPQ, independent from state transition or photo-inhibition, had been generated in the PSII complex; this was a fast component responding to high intensity light. Glutaraldehyde (GA), commonly used to stabilize membrane protein conformations, resulted in more energy transfer to PSII reaction centers, affecting the energy absorption and dissipation process rather than the transfer process of phycobilisome (PBS). In comparison experiments with and without GA, the rapid light curves (RLCs) and fluorescence induction dynamics of the fast phase showed that excess excitation energy was dissipated by conformational change in the photosynthetic pigment proteins on the thylakoid membrane (PPPTM). Based on deconvolution of NPQ relaxation kinetics, we concluded that the fast quenching component (NPQf) was closely related to PPPTM conformational change, as it accounted for as much as 39.42% of the total NPQ. We hypothesize therefore, that NPQf induced by PPPTM conformation is an important adaptation mechanism for Microcystis blooms under high-intensity light during summer and autumn.
WOS关键词PHOTOSYSTEM-II FLUORESCENCE ; ORANGE CAROTENOID PROTEIN ; SYNECHOCYSTIS PCC 6803 ; CHLOROPHYLL FLUORESCENCE ; ENERGY-DISSIPATION ; PORPHYRIDIUM-CRUENTUM ; ELECTRON-TRANSPORT ; MICROCYSTIS BLOOM ; RED ALGA ; MECHANISM
WOS研究方向Life Sciences & Biomedicine - Other Topics
语种英语
WOS记录号WOS:000308127100010
资助机构National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001] ; National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001] ; National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001] ; National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001]
公开日期2013-01-09
源URL[http://ir.ihb.ac.cn/handle/342005/17254]  
专题水生生物研究所_水环境工程研究中心_期刊论文
通讯作者Li, DH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wang ZhiCong,Dong Jing,Li DunHai,et al. Conformational changes in photosynthetic pigment proteins on thylakoid membranes can lead to fast non-photochemical quenching in cyanobacteria[J]. SCIENCE CHINA-LIFE SCIENCES,2012,55(8):726-734.
APA Wang ZhiCong,Dong Jing,Li DunHai,&Li, DH .(2012).Conformational changes in photosynthetic pigment proteins on thylakoid membranes can lead to fast non-photochemical quenching in cyanobacteria.SCIENCE CHINA-LIFE SCIENCES,55(8),726-734.
MLA Wang ZhiCong,et al."Conformational changes in photosynthetic pigment proteins on thylakoid membranes can lead to fast non-photochemical quenching in cyanobacteria".SCIENCE CHINA-LIFE SCIENCES 55.8(2012):726-734.

入库方式： OAI收割

来源：水生生物研究所

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