Effect of culture condition on the growth, biochemical composition and EPA production of alkaliphilic Nitzschia plea isolated in the Southeast of China
文献类型：期刊论文
| 作者 | Zhang, Dongmei; Wen, Shumei; Wu, Xia; Cong, Wei |
| 刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 41期号:6页码:831-839 |
| 关键词 | Alkaliphilic microalgae Eicosapntemacnioc acid Nitzschia plea Response surface |
| ISSN号 | 1615-7591 |
| DOI | 10.1007/s00449-018-1917-0 |
| 英文摘要 | To overcome the contamination in open pond, microalgal strain selection should focus on species with tolerability to extreme environments. In this study, a native alkaliphilic algae, diatom Nitzschia plea was obtained in Southeast of China, which could tolerate high concentration of NaHCO3 (0.15 mol/L) and high pH (> 10). The effects of initial pH, light intensity and temperature on cell growth, biochemical composition and fatty acid profile of N. plea were investigated. Results indicated its specific growth rate could reach 1.2 day(-1), lipid content was in the range 14.6-30.2% of dry weight, eicosapntemacnioc acid (EPA, C20:5) accounted for around 15% of total fatty acids. Alkalic condition benefited for both cell growth and EPA synthesis. Appropriately increasing light intensity and temperature could improve cell growth rate and lipid synthesis, although the proportion of EPA in total fatty acids decreased slightly. The optimal culture condition (pH 9.00, temperature 35.0 A degrees C, light intensity 158.6 A mu mol/m(2)s) was suggested for maximum yield of EPA based on the response surface model. The overall biomass productivity and EPA productivity were 0.301 g/L/day and 7.43 mg/L/day, respectively. In conclusion, alkalic environment was helpful for the steady operation of open pond cultivation of N. plea with the characteristics of fast growth rate and high EPA content, which exhibited its commercial value. |
| WOS关键词 | SPIRULINA-PLATENSIS ; CHLORELLA-VULGARIS ; MARINE MICROALGAE ; CARBON-DIOXIDE ; TEMPERATURE ; ACCUMULATION ; BICARBONATE ; PURIFICATION ; DUNALIELLA ; DIVERSITY |
| 资助项目 | National Key R&D Program of China[2016YF0601005] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000432600500009 |
| 出版者 | SPRINGER |
| 资助机构 | National Key R&D Program of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/24617]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Cong, Wei |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dongmei,Wen, Shumei,Wu, Xia,et al. Effect of culture condition on the growth, biochemical composition and EPA production of alkaliphilic Nitzschia plea isolated in the Southeast of China[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2018,41(6):831-839. |
| APA | Zhang, Dongmei,Wen, Shumei,Wu, Xia,&Cong, Wei.(2018).Effect of culture condition on the growth, biochemical composition and EPA production of alkaliphilic Nitzschia plea isolated in the Southeast of China.BIOPROCESS AND BIOSYSTEMS ENGINEERING,41(6),831-839. |
| MLA | Zhang, Dongmei,et al."Effect of culture condition on the growth, biochemical composition and EPA production of alkaliphilic Nitzschia plea isolated in the Southeast of China".BIOPROCESS AND BIOSYSTEMS ENGINEERING 41.6(2018):831-839. |
入库方式： OAI收割
来源：过程工程研究所
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