Toward understanding the key enzymes involved in beta-poly (L-malic acid) biosynthesis by Aureobasidium pullulans ipe-1
文献类型:期刊论文
作者 | Yu, Haifeng1,2; Liu, Bin1,2; Luo, Jianquan1,3; Cao, Weifeng1; Qiao, Changsheng4; Wan, Yinhua1,3 |
刊名 | ENGINEERING IN LIFE SCIENCES |
出版日期 | 2018-06-01 |
卷号 | 18期号:6页码:379-386 |
ISSN号 | 1618-0240 |
关键词 | Aureobasidium pullulans Biosynthesis mechanism beta-poly (L-malic acid) Glucose-6-phosphate dehydrogenase Phosphoenolpyruvate carboxylase |
DOI | 10.1002/elsc.201700209 |
英文摘要 | beta-poly (L-malic acid) (PMLA) is a biopolyester which has attracted industrial interest for its potential application in medicine and other industries. A high dissolved oxygen concentration (DO) was beneficial for PMLA production, while the mechanisms of DO in PMLA biosynthesis by Aureobasidium pullulans are still poorly understood. In this work, the amount of PMLA was first compared when A. pullulans ipe-1 were cultured under a high DO level (70% saturation) and a low DO level (10% saturation). Meanwhile, the key enzymes involved in different pathways of the precursor L-malic acid biosynthesis were studied. The results revealed that the activities of glucose-6-phosphate dehydrogenase (G6PDH) and phosphoenolpyruvate carboxylase (PEPC) were positively correlated with cell growth and PMLA production, while the activities of phosphofructokinases (PFK), pyruvic carboxylase (PC) and citrate synthetase (CS) did no show such correlations. It indicated that the Pentose Phosphate Pathway (PPP) may play a vital role in cell growth and PMLA biosynthesis. Moreover, the precursor L-malic acid for PMLA biosynthesis was mainly biosynthesized through phosphoenolpyruvic acid (PEP) via oxaloacetate catalyzed by PEPC. It was also found that low concentration of sodium fluoride (NaF) might impel carbon flux flow to the oxaloacetate through PEP, but inhibit the flux to the oxaloacetate via pyruvic acid. |
WOS关键词 | DISSOLVED-OXYGEN CONCENTRATION ; PHYSARUM-POLYCEPHALUM ; CONTROL STRATEGY ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; SACCHAROMYCES-CEREVISIAE ; ENHANCED PRODUCTION ; ESCHERICHIA-COLI ; POLY(MALIC ACID) ; BATCH CULTURE ; BETA-POLY(L-MALATE) |
资助项目 | Beijing Natural Science Foundation[5182025] ; National Natural Science Foundation of China[21406240] ; National High Technology Research and Development Program of China[2015AA021002] ; National High Technology Research and Development Program of China[2014AA021005] |
WOS研究方向 | Biotechnology & Applied Microbiology |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000434352900005 |
资助机构 | Beijing Natural Science Foundation ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China |
源URL | [http://ir.ipe.ac.cn/handle/122111/24710] |
专题 | 中国科学院过程工程研究所 |
通讯作者 | Wan, Yinhua |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Qilu Univ Technol, Coll Food Sci & Engn, Jinan, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing, Peoples R China 4.Tianjin Univ Sci & Technol, Coll Bioengn, Tianjin, Peoples R China |
推荐引用方式 GB/T 7714 | Yu, Haifeng,Liu, Bin,Luo, Jianquan,et al. Toward understanding the key enzymes involved in beta-poly (L-malic acid) biosynthesis by Aureobasidium pullulans ipe-1[J]. ENGINEERING IN LIFE SCIENCES,2018,18(6):379-386. |
APA | Yu, Haifeng,Liu, Bin,Luo, Jianquan,Cao, Weifeng,Qiao, Changsheng,&Wan, Yinhua.(2018).Toward understanding the key enzymes involved in beta-poly (L-malic acid) biosynthesis by Aureobasidium pullulans ipe-1.ENGINEERING IN LIFE SCIENCES,18(6),379-386. |
MLA | Yu, Haifeng,et al."Toward understanding the key enzymes involved in beta-poly (L-malic acid) biosynthesis by Aureobasidium pullulans ipe-1".ENGINEERING IN LIFE SCIENCES 18.6(2018):379-386. |
入库方式: OAI收割
来源:过程工程研究所
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