Owing to the extensive applications of hydrogen peroxide (H2O2) in biological, environmental and chemical engineering, it is of great importance to investigate sensitive and selective sensing platform towards the detection of H2O2. Herein, oxygen-implanted MoS2 nanoparticles decorated graphene nanocomposite is synthesized via a facile one-pot solvothermal method for the sensitive detection of H2O2 in alkaline media. The structure and morphology of the MoS2/graphene nanocomposites were systematically characterized, showing that Mo-O bonds are formed and oxygen is implanted into the crystal structure in the nanocomposite. As a result, the MoS2/graphene composite exhibited enhanced electron transfer kinetics and excellent electro-reduction performance towards 11202 in alkaline media. Under optimum conditions, the fabricated sensor demonstrated a wide linear response towards H2O2 in the range of 0.25-16 mM with a low detection limit of 0.12 mu M and high sensitivity of 269.7 mu A mM(-1) cm(-2). Besides, the constructed sensor presented a good selectivity to H2O2 with the presence of other interfering species. Therefore, the proposed sensor was successfully applied for the detection and determination of H2O2 in real sample, indicating great potential for the practical applications.