Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production
文献类型：期刊论文
| 作者 | Samak, Nadia A.1,2,3; Hu, Jianhua1,2,3; Wang, Kefeng1,2,3; Guo, Chen1,2; Liu, Chunzhao1,2 |
| 刊名 | WASTE AND BIOMASS VALORIZATION
 |
| 出版日期 | 2018-03-01 |
| 卷号 | 9期号:3页码:369-377 |
| 关键词 | Bacillus Subtilis Bacterial Laccase Cota Iptg Micro-aerobic Condition Ros |
| ISSN号 | 1877-2641 |
| DOI | 10.1007/s12649-016-9824-6 |
| 文献子类 | Article |
| 英文摘要 | Recently, bacterial laccases has drawn researchers' interest due to their ability to overcome high pH and salt concentration conditions compared to fungal laccases. Here we report a novel micro-aerobic cultivation strategy for enhancing CotA laccase expression and study its application for dye decolorization. Micro-aerobic cultivation of Escherichia coli BL21 (DE3) strain carrying pT7-FLAG-MAT-TAG-1-CotA had significantly enhanced CotA laccase activity up to 13903 U/L. The most unique findings of this investigation are that micro-aerobic cultivation strategy enhanced the reactive oxygen species production which consequently led to the over expression of CotA laccase gene. Malachite green, Crystal violet, Congo red and Bromophenol blue were efficiently decolorized by using purified CotA laccase without presence of any mediators at pH 6 and 9. These results provide a great platform for the dynamic production and application of bacterial laccase in industry. |
| WOS关键词 | RECOMBINANT ESCHERICHIA-COLI ; GENE-EXPRESSION ; BACILLUS-LICHENIFORMIS ; OXIDATIVE STRESS ; WASTE-WATER ; DECOLORIZATION ; CLONING ; DYES ; PROTEINS ; ACCUMULATION |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000425332300004 |
| 资助机构 | National Key Technology Research and Development Program of China(2015BAK45B01) ; National Natural Science Foundation of China(21476242) ; National Basic Research Program (973 Program) of China(2013CB733600) ; Chinese Government |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/24027]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Samak, Nadia A.,Hu, Jianhua,Wang, Kefeng,et al. Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production[J]. WASTE AND BIOMASS VALORIZATION,2018,9(3):369-377. |
| APA | Samak, Nadia A.,Hu, Jianhua,Wang, Kefeng,Guo, Chen,&Liu, Chunzhao.(2018).Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production.WASTE AND BIOMASS VALORIZATION,9(3),369-377. |
| MLA | Samak, Nadia A.,et al."Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production".WASTE AND BIOMASS VALORIZATION 9.3(2018):369-377. |
入库方式： OAI收割
来源：过程工程研究所
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