Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs
文献类型：期刊论文
| 作者 | Zhang, Jiabin1; Yun, Seonho1; Bi, Jingxiu1; Dai, Sheng1; Du, Yuguang2; Zannettino, Andrew C. W.3; Zhang, Hu1 |
| 刊名 | JOURNAL OF MATERIALS CHEMISTRY B
 |
| 出版日期 | 2018-03-28 |
| 卷号 | 6期号:12页码:1799-1814 |
| ISSN号 | 2050-750X |
| DOI | 10.1039/c8tb00376a |
| 文献子类 | Article |
| 英文摘要 | Human mesenchymal stem/stromal cells (hMSCs) are a potential cell source of stem cell therapy for many serious diseases and hMSC spheroids have emerged to replace single cell suspensions for cell therapy. Three-dimensional (3D) scaffolds or hydrogels which can mimic properties of the extracellular matrix (ECM) have been widely explored for their application in tissue regeneration. However, there are considerably less studies on inducing differentiation of hMSC spheroids using 3D scaffolds or hydrogels. This study is the first to explore multi-lineage differentiation of a stem cell line and primary stem cells within poly(N-isopropylacrylamide) (p(NIPAAm))-based thermosensitive microgel-formed constructs. We first demonstrated that poly(N-isopropylacrylamide-co-acrylic acid) (p(NIPAAm-AA)) was not toxic to hMSCs and the microgel-formed constructs facilitated formation of uniform stem cell spheroids. Due to functional enhancement of cell spheroids, hMSCs within the 3D microgel-formed constructs were induced for multi-lineage differentiation as evidenced by significant up-regulation of messenger RNA (mRNA) expression of chondrogenic and osteogenic genes even in the absence of induction media on day 9. When induction media were in situ supplied on day 9, mRNA expression of chondrogenic, osteogenic and adipogenic genes within the microgel-formed constructs were significantly higher than that in the pellet and 2D cultures, respectively, on day 37. In addition, histological and immunofluorescent images also confirmed successful multi-lineage differentiation of hMSCs within the 3D microgel-formed constructs. Hence, the thermosensitive p(NIPAAm-AA) microgel can be potentially used in an in vitro model for cell differentiation or in vivo transplantation of pre-differentiated human mesenchymal stromal cells into patients for specific lineage differentiation. |
| WOS关键词 | INTERVERTEBRAL DISC REGENERATION ; STEM-CELLS ; PRECURSOR CELLS ; STROMAL CELLS ; OSTEOGENIC DIFFERENTIATION ; PENTOSAN POLYSULFATE ; INJECTABLE HYDROGEL ; OVINE MODEL ; REPAIR ; CULTURES |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000428986900009 |
| 资助机构 | IPE-UoA Scholarship (BES) ; ARC Discovery Project(DP160104632) ; Medical Advancement Without Animal (MAWA) Trust |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/24177]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Univ Adelaide, Sch Chem Engn, Adelaide, SA 5005, Australia 2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 3.Univ Adelaide, Adelaide Med Sch, Adelaide, SA 5001, Australia |
| 推荐引用方式 GB/T 7714 | Zhang, Jiabin,Yun, Seonho,Bi, Jingxiu,et al. Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs[J]. JOURNAL OF MATERIALS CHEMISTRY B,2018,6(12):1799-1814. |
| APA | Zhang, Jiabin.,Yun, Seonho.,Bi, Jingxiu.,Dai, Sheng.,Du, Yuguang.,...&Zhang, Hu.(2018).Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs.JOURNAL OF MATERIALS CHEMISTRY B,6(12),1799-1814. |
| MLA | Zhang, Jiabin,et al."Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs".JOURNAL OF MATERIALS CHEMISTRY B 6.12(2018):1799-1814. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。