Stereospecificity of Enoylreductase Domains from Modular Polyketide Synthases
文献类型：期刊论文
| 作者 | Zhang, Luyun1,2; Ji, Junjie3; Yuan, Meijuan1,2; Feng, Yuanyuan1,2; Wang, Lei1,2; Deng, Zixin1,2; Bai, Linquan1,2; Zheng, Jianting1,2 |
| 刊名 | ACS CHEMICAL BIOLOGY
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 13期号:4页码:871-875 |
| ISSN号 | 1554-8929 |
| DOI | 10.1021/acschembio.7b00982 |
| 文献子类 | Article |
| 英文摘要 | An enoylreductase (ER) domain of a polyketide synthase module recruiting a methylmalonate extender unit sets the C2 methyl branch to either the S or R configuration during processing of a polyketide intermediate carried by an acyl carrier protein (ACP) domain. In the present study, pantetheine- and ACP-bound trans-2-methylcrotonyl substrate surrogates were used to scrutinize the stereospecificity of the ER domains. The pantetheine-bound thioester was reduced to mixtures of both 2R and 2S products, whereas the expected 2S epimer was almost exclusively generated when the cognate ACP-bound substrate surrogate was utilized. The analogous incubation of an ER with the substrate surrogate carried by a noncognate ACP significantly increased the generation of the unexpected 2R epimer, highlighting the dependence of stereospecificity on proper protein protein interactions between ER and ACP domains. The ER mutant assays revealed the involvement of the conserved tyrosine and lysine in stereocontrol. Taken together, these results expand the current understanding of the ER stereochemistry and help in the engineering of modular PKSs. |
| WOS关键词 | KETOREDUCTASE DOMAINS ; CARRIER PROTEIN ; STEREOCHEMISTRY ; BIOSYNTHESIS ; CATALYSIS ; ACYLTRANSFERASE ; MUTAGENESIS ; SUBSTRATE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000430898600004 |
| 资助机构 | National Natural Science Foundation of China(31370101 ; National Program on Key Basic Research Project (973 program)(2013CB734002) ; State Key Laboratory of Pathogen and Biosecurity (Academy of Military Medical Science)(SKLPBS1526) ; Fundamental Research Funds for the Central Universities ; Global Talents Recruitment Program ; 31570056 ; 31770068) |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/24436]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Luyun,Ji, Junjie,Yuan, Meijuan,et al. Stereospecificity of Enoylreductase Domains from Modular Polyketide Synthases[J]. ACS CHEMICAL BIOLOGY,2018,13(4):871-875. |
| APA | Zhang, Luyun.,Ji, Junjie.,Yuan, Meijuan.,Feng, Yuanyuan.,Wang, Lei.,...&Zheng, Jianting.(2018).Stereospecificity of Enoylreductase Domains from Modular Polyketide Synthases.ACS CHEMICAL BIOLOGY,13(4),871-875. |
| MLA | Zhang, Luyun,et al."Stereospecificity of Enoylreductase Domains from Modular Polyketide Synthases".ACS CHEMICAL BIOLOGY 13.4(2018):871-875. |
入库方式： OAI收割
来源：过程工程研究所
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