Three-dimensional aerators are often used in hydraulic structures to prevent cavitation damage via enhanced air entrainment. However, the mechanisms of aeration and bubble dispersion along the developing shear flow region on such aerators remain unclear. A double-tip conductivity probe is employed in present experimental study to investigate the air concentration, bubble count rate, and bubble size downstream of a three-dimensional aerator involving various approach-flow features and geometric parameters. The results show that the cross-sectional distribution of the air bubble frequency is in accordance with the Gaussian distribution, and the relationship between the air concentration and bubble frequency obeys a quasi-parabolic law. The air bubble frequency reaches an apex at an air concentration (C) of approximately 50% and decreases to zero as C = 0% and C = 100%. The relative location of the air-bubble frequency apex is 0.210, 0.326 and 0.283 times the thickness of the layers at the upper, lower and sdenappes, respectively. The air bubble chord length decreases gradually from the air water interface to the core area. The air concentration increases exponentially with the bubble chord length. The air bubble frequency distributions can be fit well using a “modified” gamma distribution function.