Enhanced catalytic stability of lipase immobilized on oxidized and disulfide-rich eggshell membrane for esters hydrolysis and transesterification
文献类型：期刊论文
| 作者 | Shen, Cai; Jiang, Chenyu; Cheng, Chuanchuan; Hao, Mei; Wang, Hongbin; Wang, Ziying; Cheong, Ling-Zhi |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2017 |
| 卷号 | 105页码:1328-1336 |
| ISSN号 | 0141-8130 |
| 英文摘要 | Eggshell membrane (ESM) is an industrial waste that is available in abundance from food industry. Present study investigated the physicochemical properties of oxidized ESM and compared the efficiency of ESM and oxidized ESM as carrier for Burkholderia cepacia lipase (BCL) used in esters hydrolysis and transesterification. Following oxidation treatment, FTIR analysis and Ellman's assay showed amino acid cysteine in ESM was oxidized to form disulfide bond-containing cystine. In addition, AFM analysis showed ESM which exhibited a highly porous filamentous structure appeared to be coalesce following oxidation treatment. Oxidized ESM also showed reduced porosity (38.67%) in comparison to native ESM (51.65%). BCL were successfully immobilized on oxidized ESM through carrier activation method (enzyme loading of 5.01 mg protein/g oxidized ESM). These immobilized lipase demonstrated significantly (P < 0.05) enhanced catalytic stability with close to 100% of initial hydrolysis (12.03 +/- 0.29 mmol/min/g) activity; and more than 85% of its initial transesterification (7.83 +/- 0.05) activity for at least 10 consecutive runs. Enhanced catalytic stability of BCL immobilized on oxidized ESM might be due to stabilization of the protein structure in oxidized ESM by disulfide bonds which helped formation of a stable bonding with BCL. (C) 2017 Elsevier B.V. All rights reserved. |
| 公开日期 | 2017-12-25 |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/13484]  |
| 专题 | 2017专题 |
| 推荐引用方式 GB/T 7714 | Shen, Cai,Jiang, Chenyu,Cheng, Chuanchuan,et al. Enhanced catalytic stability of lipase immobilized on oxidized and disulfide-rich eggshell membrane for esters hydrolysis and transesterification[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017,105:1328-1336. |
| APA | Shen, Cai.,Jiang, Chenyu.,Cheng, Chuanchuan.,Hao, Mei.,Wang, Hongbin.,...&Cheong, Ling-Zhi.(2017).Enhanced catalytic stability of lipase immobilized on oxidized and disulfide-rich eggshell membrane for esters hydrolysis and transesterification.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,105,1328-1336. |
| MLA | Shen, Cai,et al."Enhanced catalytic stability of lipase immobilized on oxidized and disulfide-rich eggshell membrane for esters hydrolysis and transesterification".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 105(2017):1328-1336. |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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