A Targeted and Stable Polymeric Nanoformulation Enhances Systemic Delivery of mRNA to Tumors
文献类型：期刊论文
| 作者 | Dong, Wenfei; Chen, Qixian; Qi, Ruogu; Chen, Xiyi; Yang, Xi; Wu, Sudong; Xiao, Haihua |
| 刊名 | MOLECULAR THERAPY
 |
| 出版日期 | 2017 |
| 卷号 | 25期号:1页码:92-101 |
| ISSN号 | 1525-0016 |
| 英文摘要 | The high vulnerability of mRNA necessitates the manufacture of delivery vehicles to afford adequate protection in the biological milieu. Here, mRNA was complexed with a mixture of cRGD-poly(ethylene glycol) (PEG)-polylysine (PLys) (thiol) and poly(N-isopropylacrylamide) (PNIPAM)-PLys(thiol). The ionic complex core consisting of opposite-charged PLys and mRNA was crosslinked though redox-responsive disulfide linkage, thereby avoiding structural disassembly for exposure of mRNA to harsh biological environments. Furthermore, PNIPAM contributed to prolonged survival in systemic circulation by presenting a spatial barrier in impeding accessibility of nucleases, e.g., RNase, due to the thermo-responsive hydrophilic-hydrophobic transition behavior upon incubation at physiological temperature enabling translocation of PNIPAM from shell to intermediate barrier. Ultimately, the cRGD ligand attached to the formulation demonstrated improved tumor accumulation and potent gene expression, as manifested by virtue of facilitated cellular uptake and intracellular trafficking. These results indicate promise for the utility of mRNA as a therapeutic tool for disease treatment. |
| 公开日期 | 2017-12-25 |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/14045]  |
| 专题 | 2017专题 |
| 推荐引用方式 GB/T 7714 | Dong, Wenfei,Chen, Qixian,Qi, Ruogu,et al. A Targeted and Stable Polymeric Nanoformulation Enhances Systemic Delivery of mRNA to Tumors[J]. MOLECULAR THERAPY,2017,25(1):92-101. |
| APA | Dong, Wenfei.,Chen, Qixian.,Qi, Ruogu.,Chen, Xiyi.,Yang, Xi.,...&Xiao, Haihua.(2017).A Targeted and Stable Polymeric Nanoformulation Enhances Systemic Delivery of mRNA to Tumors.MOLECULAR THERAPY,25(1),92-101. |
| MLA | Dong, Wenfei,et al."A Targeted and Stable Polymeric Nanoformulation Enhances Systemic Delivery of mRNA to Tumors".MOLECULAR THERAPY 25.1(2017):92-101. |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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