In vitro preservation and micropropagation of Oreocharis mileense (WT Wang) M. Moller & A. Weber (Gesneriaceae) through shoot organogenesis
文献类型：期刊论文
| 作者 | Wang, Dandan1; Li, Xinhui2 ; Cheng, Zhiying3,4; Long, Chunlin3,4,5
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| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
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| 出版日期 | 2018-12-01 |
| 卷号 | 54期号:6页码:606-611 |
| ISSN号 | 1054-5476 |
| 关键词 | Oreocharis mileense Rediscovery Critically endangered In vitro preservation Micropropagation |
| 英文摘要 | Oreocharis mileense (W.T. Wang) M. Moller & A. Weber is endemic to China and was considered to be extinct because it had not been seen in the wild since the first collection in 1906. In 2006, the species was rediscovered in Shilin County, Yunnan Province. Oreocharis mileense was considered critically endangered for its narrow geographic range and extremely small population. An efficient method to preserve plant germplasm by in vitro culturing of O. mileense has not been reported. In this study, an orthogonal array with three factors (6-benzyladenine, BA; alpha-naphthaleneacetic acid, NAA; and sucrose), at four levels was performed, and shoot induction as well as shoot proliferation were recorded. The results were analyzed to determine the most significant components and the optimum combination for micropropagation of O. mileense. The results showed that: (1) organogenesis was easily induced by different combinations of plant-growth regulators and sucrose; (2) NAA and sucrose had the most significant effect on shoot induction and shoot multiplication, and (3) the optimum induction and proliferation media were 0.5 mg L-1 BA + 0.2 mg L-1 NAA + 30 g L-1 sucrose and 1 mg L-1 BA + 0.1 mg L-1 NAA + 30 g L-1 sucrose, respectively. |
| 资助项目 | Ministry of Science and Technology of China[2005DKA21006] ; Ministry of Science and Technology of China[2012FY110300] ; National Natural Science Foundation of China[31761143001] ; National Natural Science Foundation of China[31870316] ; National Natural Science Foundation of China[31070288] ; National Natural Science Foundation of China[31560063] ; Ministry of Education of China[yldxxk201819] ; Ministry of Education of China[2015MDTD16C] ; Ministry of Education of China[B08044] ; Key Laboratory of Ethnomedicine (Minzu University of China) of the Ministry of Education of China[KLEM-ZZ201806] |
| 语种 | 英语 |
| WOS记录号 | WOS:000450664800005 |
| 资助机构 | Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Ministry of Education of China ; Key Laboratory of Ethnomedicine (Minzu University of China) of the Ministry of Education of China |
| 源URL | [http://ir.kib.ac.cn/handle/151853/63282]  |
| 专题 | 中国科学院昆明植物研究所 |
| 通讯作者 | Long, Chunlin |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 2.Southwest Forestry Univ, Coll Ecol & Soil & Water Conservat, Kunming 650224, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resource, Kunming 650201, Yunnan, Peoples R China 5.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dandan,Li, Xinhui,Cheng, Zhiying,et al. In vitro preservation and micropropagation of Oreocharis mileense (WT Wang) M. Moller & A. Weber (Gesneriaceae) through shoot organogenesis[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2018,54(6):606-611. |
| APA | Wang, Dandan,Li, Xinhui,Cheng, Zhiying,&Long, Chunlin.(2018).In vitro preservation and micropropagation of Oreocharis mileense (WT Wang) M. Moller & A. Weber (Gesneriaceae) through shoot organogenesis.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,54(6),606-611. |
| MLA | Wang, Dandan,et al."In vitro preservation and micropropagation of Oreocharis mileense (WT Wang) M. Moller & A. Weber (Gesneriaceae) through shoot organogenesis".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 54.6(2018):606-611. |
入库方式： OAI收割
来源：昆明植物研究所
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