1,4-beta-D-Glucomannan from Dendrobium officinale Activates NF-kappa B via TLR4 to Regulate the Immune Response
文献类型：期刊论文
| 作者 | Huang, Yan-Ping1,2,3; He, Tao-Bin1,2,3,4; Cuan, Xian-Dan1,2,3; Wang, Xuan-Jun1,2,3; Hu, Jiang-Miao4 ; Sheng, Jun1,2,3
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| 刊名 | MOLECULES
 |
| 出版日期 | 2018-10-01 |
| 卷号 | 23期号:10页码:15 |
| 关键词 | Dendrobium officinale 1,4-beta-D-glucomannan thermal stability NF-kappa B TLR4 |
| ISSN号 | 1420-3049 |
| 英文摘要 | 2,3-O-acetylated-1,4-beta-D-glucomannan (DOP-1-1) is a polysaccharide isolated from the stem of Dendrobium officinale. DOP-1-1 has been demonstrated to have remarkable immunomodulatory properties, but little is known about the influence of its structural diversity on bioactivity (and even less about the exact mechanism underlying its immune responses). First, DOP-1-1 was stabilized at different temperatures and pH conditions based on differential scanning calorimetry and size exclusion-chromatography-high-performance liquid chromatography. Then, a detailed study on the effects of DOP-1-1 on a human leukemia monocytic cell line (THP-1) under normal conditions was undertaken. DOP-1-1 promoted the translocation of nuclear factor-kappa B (NF-kappa B) and degradation of I kappa B proteins. The expression of genes and proteins closely associated with the immune, survival and apoptotic functions of NF-kappa B were analyzed by quantitative real-time RT-PCR. Furthermore, CCL4 and IP10 were confirmed to be the novel targets of the immune response stimulated by DOP-1-1. The phosphorylation of NF-kappa B was inhibited by treatment with a toll-like receptor 4 (TLR4) antagonist (TAK-242) and myeloid differentiation factor 88 (MyD88) inhibitor (ST2825). These data suggested: (i) the O-acetylated glucomannan DOP-1-1 is present in the steady state in low-pH solutions; (ii) DOP-1-1 can induce an immune response through NF-kappa B mediated by a TLR4 signaling pathway; and (iii) CCL4 and IP10 could be the novel targets of the immune response stimulated by O-acetylated glucomannan. |
| 资助项目 | Natural Science Foundation of Yunnan Province, China[2012FB151] ; Foundation of Scientific and Technical Innovation of Yunnan Province, China[2009AB021] ; Foundation of Scientific and Technical Innovation of Yunnan Province, China[2017ZF003] ; Yunnan Agricultural University Innovation Foundation for Postgraduates[2016ykc25] |
| 语种 | 英语 |
| WOS记录号 | WOS:000451201400254 |
| 资助机构 | Natural Science Foundation of Yunnan Province, China ; Foundation of Scientific and Technical Innovation of Yunnan Province, China ; Yunnan Agricultural University Innovation Foundation for Postgraduates |
| 源URL | [http://ir.kib.ac.cn/handle/151853/63300]  |
| 专题 | 中国科学院昆明植物研究所 |
| 通讯作者 | Wang, Xuan-Jun; Hu, Jiang-Miao; Sheng, Jun |
| 作者单位 | 1.Yunnan Agr Univ, Minist Educ, Key Lab Pu Er Tea Sci, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Agr Univ, Coll Sci, Kunming 650201, Yunnan, Peoples R China 3.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Yan-Ping,He, Tao-Bin,Cuan, Xian-Dan,et al. 1,4-beta-D-Glucomannan from Dendrobium officinale Activates NF-kappa B via TLR4 to Regulate the Immune Response[J]. MOLECULES,2018,23(10):15. |
| APA | Huang, Yan-Ping,He, Tao-Bin,Cuan, Xian-Dan,Wang, Xuan-Jun,Hu, Jiang-Miao,&Sheng, Jun.(2018).1,4-beta-D-Glucomannan from Dendrobium officinale Activates NF-kappa B via TLR4 to Regulate the Immune Response.MOLECULES,23(10),15. |
| MLA | Huang, Yan-Ping,et al."1,4-beta-D-Glucomannan from Dendrobium officinale Activates NF-kappa B via TLR4 to Regulate the Immune Response".MOLECULES 23.10(2018):15. |
入库方式： OAI收割
来源：昆明植物研究所
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