Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe
文献类型：期刊论文
| 作者 | Liu, Maili3; Zhang, Xu3; Cao, Chunyang1; Lan, Wenxian1; Li, Conggang3; Jiang, Bin3; Zhu, Qinjun3; Yuan, Bin3; Wang, Qianwen2,3; Sun, Peng3 |
| 刊名 | CHEMICAL COMMUNICATIONS
 |
| 出版日期 | 2018-11-18 |
| 卷号 | 54期号:89页码:12630-12633 |
| ISSN号 | 1359-7345 |
| DOI | 10.1039/c8cc07605g |
| 英文摘要 | Spectral overlap makes it difficult to use NMR for mapping the conformational profile of heterogeneous conformational ensembles of macromolecules. Here, we apply a H-1-N-14 HSQC experiment to monitor the alkaline conformational transitions of yeast iso-1 cytochrome c (ycyt c) at natural isotopic abundance. Trimethylated Lys72 of ycyt c is selectively detected by a H-1-N-14 HSQC experiment, and used as a probe to trace conformational transitions of ycyt c under alkaline conditions. It was found that at least four different conformers of ycyt c coexisted under alkaline conditions. Besides the native structure, Lys73 or Lys79 coordinated conformers and a partially unfolded state with exposed heme were observed. These results indicate that the method is powerful at simplifying spectra of a trimethylated protein, which makes it possible to study complex conformational transitions of naturally extracted or chemically modified trimethylated protein at natural isotopic abundance. |
| WOS关键词 | CHOLINE-CONTAINING COMPOUNDS ; FERRICYTOCHROME-C ; LIVING CELLS ; CONFORMATIONAL TRANSITIONS ; PEROXIDASE-ACTIVITY ; SPECTROSCOPY ; PROTEINS ; STATE ; ISO-1-CYTOCHROME-C ; IDENTIFICATION |
| 资助项目 | National Key R&D Program of China[2017YFA0505400] ; National Natural Science Foundation of China[21735007] ; National Natural Science Foundation of China[21505153] ; National Natural Science Foundation of China[21675170] ; National Natural Science Foundation of China[21475146] ; National Natural Science Foundation of China[21773300] ; CAS Key Research Program of Frontier Sciences[QYZDJ-SSW-SLH027] ; K. C. Wong Educational Foundation |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000449274400015 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | National Key R&D Program of China ; National Natural Science Foundation of China ; CAS Key Research Program of Frontier Sciences ; K. C. Wong Educational Foundation ; National Key R&D Program of China ; National Natural Science Foundation of China ; CAS Key Research Program of Frontier Sciences ; K. C. Wong Educational Foundation ; National Key R&D Program of China ; National Natural Science Foundation of China ; CAS Key Research Program of Frontier Sciences ; K. C. Wong Educational Foundation ; National Key R&D Program of China ; National Natural Science Foundation of China ; CAS Key Research Program of Frontier Sciences ; K. C. Wong Educational Foundation |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/13200]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 通讯作者 | Liu, Maili; Zhang, Xu |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, CAS Key Lab Magnet Resonance Biol Syst, Wuhan Natl Ctr Magnet Resonance,Wuhan Inst Phys &, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Maili,Zhang, Xu,Cao, Chunyang,et al. Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe[J]. CHEMICAL COMMUNICATIONS,2018,54(89):12630-12633. |
| APA | Liu, Maili.,Zhang, Xu.,Cao, Chunyang.,Lan, Wenxian.,Li, Conggang.,...&Sun, Peng.(2018).Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe.CHEMICAL COMMUNICATIONS,54(89),12630-12633. |
| MLA | Liu, Maili,et al."Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe".CHEMICAL COMMUNICATIONS 54.89(2018):12630-12633. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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