中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 武汉物理与数学研究所
  3. 中国科学院武汉物理与数学研究所
Vibrio alginolyticus infection induces coupled changes of bacterial community and metabolic phenotype in the gut of swimming crab

文献类型：期刊论文

作者Wang, Chunlin3; Ye, Yangfang3; Liu, Lei3; Zhang, Limin1; Mu, Changkao3; Li, Ronghua3; Xia, Mengjie2,3; Shi, Ce3
刊名AQUACULTURE
出版日期2019-01-15
卷号499页码:251-259
关键词Portunus trituberculatus Vibrio alginolyticus Gut microbiota 16S rRNA gene amplicon sequencing Metabolomics Nuclear magnetic resonance (NMR)
ISSN号0044-8486
DOI10.1016/j.aquaculture.2018.09.031
英文摘要The emulsification disease of swimming crab Portunus trituberculatus is mainly caused by Vibrio alginolyticus. Gut microbiota is involved in the immunity and diseases of crustaceans. In this study, we integrated 16S rRNA gene sequencing and H-1 nuclear magnetic resonance metabolomics techniques to determine the effects of V. alginolyticus infection on bacterial composition and metabolic phenotype in the gut of P. trituberculatus. The results showed that V. alginolyticus infection induced marked changes in the gut bacterial composition of P. trituberculatus, which seems to drive the gut bacterial community into a kind of diseased status. The sums of the relative abundance of these indicator bacteria such as Vibrionaceae and Pseudoalteromonadaceae could be used to predict the increased risk of emulsification disease occurrence in swimming crab. V. alginolyticus infection also caused marked changes in the gut metabolic phenotype of P. trituberculatus, highlighted by the significantly changed levels of maltose, glucose, AMP, and amino acids. Importantly, the gut indicator bacteria showed comprehensive correlations with these significantly changed gut metabolites, which revealing the contributions of gut bacteria in pathogenesis of gut metabolic disorders of swimming crab after V. alginolyticus infection. Overall, this study provides solid evidence that gut bacteria are involved in the occurrence and development of crab vibriosis, which could be great important for gaining new insights into the pathogenesis mechanisms of emulsification disease and developing novel strategies for emulsification disease prevention of swimming crab.
WOS关键词DAMSELAE SUBSP PISCICIDA ; PORTUNUS-TRITUBERCULATUS ; MOLECULAR-MECHANISMS ; SHRIMP DISEASE ; MICROBIOTA ; PHOTOBACTERIUM ; EXPRESSION ; GENE ; SENSITIVITY ; SEVERITY
资助项目National Natural Science Foundation of China[41673076] ; National Natural Science Foundation of China[21577169] ; Major Agriculture Program of Ningbo[2017C110007] ; China Agriculture Research System[CARS48] ; K. C. Wong Magna Fund in Ningbo University
WOS研究方向Fisheries ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:000447083800028
出版者ELSEVIER SCIENCE BV
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Agriculture Program of Ningbo ; Major Agriculture Program of Ningbo ; China Agriculture Research System ; China Agriculture Research System ; K. C. Wong Magna Fund in Ningbo University ; K. C. Wong Magna Fund in Ningbo University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Agriculture Program of Ningbo ; Major Agriculture Program of Ningbo ; China Agriculture Research System ; China Agriculture Research System ; K. C. Wong Magna Fund in Ningbo University ; K. C. Wong Magna Fund in Ningbo University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Agriculture Program of Ningbo ; Major Agriculture Program of Ningbo ; China Agriculture Research System ; China Agriculture Research System ; K. C. Wong Magna Fund in Ningbo University ; K. C. Wong Magna Fund in Ningbo University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Agriculture Program of Ningbo ; Major Agriculture Program of Ningbo ; China Agriculture Research System ; China Agriculture Research System ; K. C. Wong Magna Fund in Ningbo University ; K. C. Wong Magna Fund in Ningbo University
源URL[http://ir.wipm.ac.cn/handle/112942/13257]  
专题中国科学院武汉物理与数学研究所
通讯作者Wang, Chunlin; Ye, Yangfang
作者单位1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, 30 Xiao Hong Shan, Wuhan 430071, Hubei, Peoples R China
2.Collaborat Innovat Ctr Zhejiang Marine High Effic, 818 Fenghua Rd, Ningbo 315211, Zhejiang, Peoples R China
3.Ningbo Univ, Chinese Minist Educ, Key Lab Appl Marine Biotechnol, 818 Fenghua Rd, Ningbo 315211, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Chunlin,Ye, Yangfang,Liu, Lei,et al. Vibrio alginolyticus infection induces coupled changes of bacterial community and metabolic phenotype in the gut of swimming crab[J]. AQUACULTURE,2019,499:251-259.
APA Wang, Chunlin.,Ye, Yangfang.,Liu, Lei.,Zhang, Limin.,Mu, Changkao.,...&Shi, Ce.(2019).Vibrio alginolyticus infection induces coupled changes of bacterial community and metabolic phenotype in the gut of swimming crab.AQUACULTURE,499,251-259.
MLA Wang, Chunlin,et al."Vibrio alginolyticus infection induces coupled changes of bacterial community and metabolic phenotype in the gut of swimming crab".AQUACULTURE 499(2019):251-259.

入库方式： OAI收割

来源：武汉物理与数学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。