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Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass

文献类型：期刊论文

作者Bai, Zetao1,2,3; Qi, Tianxiong1,2,3; Liu, Yuchen1,2,3; Wu, Zhenying1,2,3; Ma, Lichao1,2,3; Liu, Wenwen1,2,3; Cao, Yingping1,2,3; Bao, Yan1,2,3; Fu, Chunxiang1,2,3
刊名PLANT BIOTECHNOLOGY JOURNAL
出版日期2018-12-01
卷号16期号:12页码:2016-2026
关键词cystathionine gamma-synthase lignin biosynthesis methionine metabolism Panicum virgatum L. (switchgrass) S-adenosylhomocysteine S-adenosylhomocysteine hydrolase S-adenosylmethionine
ISSN号1467-7644
DOI10.1111/pbi.12935
英文摘要Methionine (Met) synthesized from aspartate is a fundamental amino acid needed to produce S-adenosylmethionine (SAM) that is an important cofactor for the methylation of monolignols. As a competitive inhibitor of SAM-dependent methylation, the effect of S-adenosylhomocysteine (SAH) on lignin biosynthesis, however, is still largely unknown in plants. Expression levels of Cystathionine gamma-synthase (PvCGS) and S-adenosylhomocysteine hydrolase 1 (PvSAHH1) were down-regulated by RNAi technology, respectively, in switchgrass, a dual-purpose forage and biofuel crop. The transgenic switchgrass lines were subjected to studying the impact of SAH on lignin biosynthesis. Our results showed that down-regulation of PvCGS in switchgrass altered the accumulation of aspartate-derived and aromatic amino acids, reduced the content of SAH, enhanced lignin biosynthesis and stunted plant growth. In contrast, down-regulation of PvSAHH1 raised SAH levels in switchgrass, impaired the biosynthesis of both guaiacyl and syringyl lignins and therefore significantly increased saccharification efficiency of cell walls. This work indicates that SAH plays a crucial role in monolignol methylation in switchgrass. Genetic regulation of either PvCGS or PvSAHH1 expression in switchgrass can change intracellular SAH contents and SAM to SAH ratios and therefore affect lignin biosynthesis. Thus, our study suggests that genes involved in Met metabolism are of interest as new valuable targets for cell wall bioengineering in future.
WOS关键词CYSTATHIONINE-GAMMA-SYNTHASE ; ADENOSYL-L-METHIONINE ; PANICUM VIRGATUM L. ; METHYLMETHIONINE CYCLE ; FUNCTIONAL-ANALYSIS ; SOLUBLE METHIONINE ; BIOFUEL PRODUCTION ; POTATO PLANTS ; RNA-SEQ ; ARABIDOPSIS
资助项目'100-Talent Program of the Chinese Academy of Sciences' foundation ; National Natural Science Foundation of China[31470390] ; National Natural Science Foundation of China[31700258] ; National Key Technologies Research & Development Program-Seven major crops breeding project[2016YFD0101803] ; Science and Technology Major Project of Shandong[2016ZDJS10A04]
WOS研究方向Biotechnology & Applied Microbiology ; Plant Sciences
语种英语
WOS记录号WOS:000449663300005
出版者WILEY
资助机构'100-Talent Program of the Chinese Academy of Sciences' foundation ; National Natural Science Foundation of China ; National Key Technologies Research & Development Program-Seven major crops breeding project ; Science and Technology Major Project of Shandong
源URL[http://ir.qibebt.ac.cn/handle/337004/11007]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Fu, Chunxiang
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Shandong, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Qingdao, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Bai, Zetao,Qi, Tianxiong,Liu, Yuchen,et al. Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass[J]. PLANT BIOTECHNOLOGY JOURNAL,2018,16(12):2016-2026.
APA Bai, Zetao.,Qi, Tianxiong.,Liu, Yuchen.,Wu, Zhenying.,Ma, Lichao.,...&Fu, Chunxiang.(2018).Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass.PLANT BIOTECHNOLOGY JOURNAL,16(12),2016-2026.
MLA Bai, Zetao,et al."Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass".PLANT BIOTECHNOLOGY JOURNAL 16.12(2018):2016-2026.

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来源：青岛生物能源与过程研究所

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