中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 青岛生物能源与过程研究所
  3. 中国科学院青岛生物能源与过程研究所
Functional cell surface displaying of acetylcholinesterase for spectrophotometric sensing organophosphate pesticide

文献类型：期刊论文

作者Liang, Bo1,2,3,4; Wang, Ge1,2,5; Yan, Lu1,2; Ren, Han6; Feng, Ruirui3,4; Xiong, Zhong5; Liu, Aihua1,2,5,6
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2019-01-15
卷号279页码:483-489
ISSN号0925-4005
关键词Carp Acetylcholinesterase (AChE) Yeast Cell surface display Organophosphate pesticide detection
DOI10.1016/j.snb.2018.09.119
英文摘要The inhibition of acetylcholinesterase (AChE) activity based methods have been widely used for rapid detection of organophosphate (OP) pesticide residues. However, the sensitivity of the enzyme toward pesticides is not desirable and complex purification steps increase production cost. In this study, we optimized and synthesized the cDNA of carp AChE based on the preferred codon using Saccharomyces cerevisiae, which was ligated into expression vector pYD1. The AChE was successfully presented on yeast surface through a-agglutinin mediated display system. In shake-flask after induction with galactose in YPD medium for 24 h, this strain expressed AChE activity at a high level of 0.15 U/OD600/ml. The correct location of AChE was confirmed by immunofluorescence analysis. The whole cell catalyst was applied to visible spectrophotometry detection for OP pesticides based on inhibition rate. The inhibition rate was linear with paraoxon concentration in the range of 0.5 ng/ml-10 mu g/ml and parathion concentration in the range of 5 ng/ml-10 mu g/ml. The low detection limit was 0.136 ng/ml paraoxon and 3.72 ng/ml parathion (S/N = 3). The proposed method is applicable to analyzing OP pesticides in real samples. Therefore, the recombinant strain developed could be an efficient and affordable biomaterial for rapid and accurate detection of OP pesticide residues.
WOS关键词MICROBIAL BIOSENSOR ; SENSITIVE ACETYLCHOLINESTERASE ; NIPPOSTRONGYLUS-BRASILIENSIS ; GLUTAMATE-DEHYDROGENASE ; GLUCOSE-DEHYDROGENASE ; XYLOSE DEHYDROGENASE ; PICHIA-PASTORIS ; NERVE AGENTS ; HYDROLASE ; EXPRESSION
资助项目National Natural Science Foundation of China[81673172] ; National Natural Science Foundation of China[21475144] ; National Natural Science Foundation of China[91227116] ; National Natural Science Foundation of China[21275152] ; Major Program of Shandong Province Natural Science Foundation[ZR2018ZC0125]
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
出版者ELSEVIER SCIENCE SA
WOS记录号WOS:000449373800060
资助机构National Natural Science Foundation of China ; Major Program of Shandong Province Natural Science Foundation
源URL[http://ir.qibebt.ac.cn/handle/337004/11010]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Liu, Aihua
作者单位1.Qingdao Univ, Inst Biosensing, 308 Ningxia Rd, Qingdao 266071, Peoples R China
2.Qingdao Univ, Coll Life Sci, 308 Ningxia Rd, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China
4.Chinese Acad Sci, Key Lab Bioenergy, 189 Songling Rd, Qingdao 266101, Peoples R China
5.Qingdao Univ, Coll Chem & Chem Engn, Qingdao 266071, Peoples R China
6.Qingdao Univ, Med Coll, Sch Pharm, Dept Drug Metab & Anal, Qingdao 266021, Peoples R China
推荐引用方式
GB/T 7714		 Liang, Bo,Wang, Ge,Yan, Lu,et al. Functional cell surface displaying of acetylcholinesterase for spectrophotometric sensing organophosphate pesticide[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,279:483-489.
APA Liang, Bo.,Wang, Ge.,Yan, Lu.,Ren, Han.,Feng, Ruirui.,...&Liu, Aihua.(2019).Functional cell surface displaying of acetylcholinesterase for spectrophotometric sensing organophosphate pesticide.SENSORS AND ACTUATORS B-CHEMICAL,279,483-489.
MLA Liang, Bo,et al."Functional cell surface displaying of acetylcholinesterase for spectrophotometric sensing organophosphate pesticide".SENSORS AND ACTUATORS B-CHEMICAL 279(2019):483-489.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。