Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos
文献类型：期刊论文
| 作者 | Wang, Fan1,2; Men, Xiao1,2; Zhang, Ge1,2; Liang, Kaichao3; Xin, Yuhua3; Wang, Juan3; Li, Aijun3; Zhang, Haibo1,2 ; Liu, Haobao3,4; Wu, Lijun5
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| 刊名 | AMB EXPRESS
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| 出版日期 | 2018-11-10 |
| 卷号 | 8页码:9 |
| 关键词 | Microbial community diversity 16S rRNA gene sequencing Optimal primer sets Aging flue-cured tobaccos PICRUSt Functional profiling prediction |
| ISSN号 | 2191-0855 |
| DOI | 10.1186/s13568-018-0713-1 |
| 英文摘要 | Selection of optimal primer pairs in 16S rRNA gene sequencing is a pivotal issue in microorganism diversity analysis. However, limited effort has been put into investigation of specific primer sets for analysis of the bacterial diversity of aging flue-cured tobaccos (AFTs), as well as prediction of the function of the bacterial community. In this study, the performance of four primer pairs in determining bacterial community structure based on 16S rRNA gene sequences in AFTs was assessed, and the functions of genes were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Results revealed that the primer set 799F-1193R covering the amplification region V5V6V7 gave a more accurate picture of the bacterial community structure of AFTs, with lower co-amplification levels of chloroplast and mitochondrial genes, and more genera covered than when using the other primers. In addition, functional gene prediction suggested that the microbiome of AFTs was involved in kinds of interested pathways. A high abundance of functional genes involved in nitrogen metabolism was detected in AFTs, reflecting a high level of bacteria involved in degrading harmful nitrogen compounds and generating nitrogenous nutrients for others. Additionally, the functional genes involved in biosynthesis of valuable metabolites and degradation of toxic compounds provided information that the AFTs possess a huge library of microorganisms and genes that could be applied to further studies. All of these findings provide a significance reference for researchers working on the bacterial diversity assessment of tobacco-related samples. |
| WOS关键词 | MICROBIAL COMMUNITIES ; DIVERSITY ; MICROORGANISMS ; CHARACTERIZE ; CULTURE ; MAIZE ; PEA |
| 资助项目 | Hainan's Key Project of Research and Development Plan[ZDYF2017155] ; Yunnan Academy of Tobacco Sciences Project[2017CP01] ; Youth Innovation Promotion Association CAS[2017252] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000449927700001 |
| 出版者 | SPRINGEROPEN |
| 资助机构 | Hainan's Key Project of Research and Development Plan ; Yunnan Academy of Tobacco Sciences Project ; Youth Innovation Promotion Association CAS |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/11013]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Zhang, Haibo; Liu, Haobao; Wu, Lijun |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Natl Tobacco Corp, Hainan Prov Branch, Hainan Cigar Res Inst, Haikou 571100, Hainan, Peoples R China 4.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Shandong, Peoples R China 5.Yunnan Acad Tobacco Sci, Kunming 650106, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fan,Men, Xiao,Zhang, Ge,et al. Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos[J]. AMB EXPRESS,2018,8:9. |
| APA | Wang, Fan.,Men, Xiao.,Zhang, Ge.,Liang, Kaichao.,Xin, Yuhua.,...&Wu, Lijun.(2018).Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos.AMB EXPRESS,8,9. |
| MLA | Wang, Fan,et al."Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos".AMB EXPRESS 8(2018):9. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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