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Mechanistic Insights into Interactions between Bacterial Class I P450 Enzymes and Redox Partners

文献类型：期刊论文

作者Zhang, Wei1,2; Du, Lei1,2; Li, Fengwei2; Zhang, Xingwang2; Qu, Zepeng2,3; Hang, Lei4; Li, Zhong2,3; Sun, Jingran2; Qi, Fengxia2; Yao, Qiuping2
刊名ACS CATALYSIS
出版日期2018-11-01
卷号8期号:11页码:9992-10003
关键词cytochrome P450 enzymes redox partners electron transfer protein protein interaction ferredoxin ferredoxin reductase
ISSN号2155-5435
DOI10.1021/acscatal.8b02913
英文摘要Cytochrome P450 enzymes are highly diversified biocatalysts associated with steroid biosynthesis, xenobiotic metabolism, biosynthesis of natural products, and industrial oxidation reactions. A typical P450 catalytic cycle requires sequential transfer of two electrons from NAD(P)H to the heme-iron reactive center for O-2 activation. For the most abundant bacterial Class I P450 systems, this important process is usually mediated by two redox partner proteins including an FAD-containing ferredoxin reductase (FdR) and a small iron sulfur protein, ferredoxin (Fdx). However, it is often unclear which pair of Fdx and FdR among multiple redox partners is the optimal one for a specific Class I P450 enzyme. To address this important but underexplored question, herein, a reaction matrix network with 16 Fdxs, 8 FdRs, and 6 P450s (against 7 substrates) was constituted. By analyzing the reactivity profiles of 896 P450 reactions, together with phylogenetic analysis, redox potential measurements, structural simulations, and Fdx-P450 molecular docking, we provide important mechanistic insights into the recognition and interactions between bacterial Class I P450 enzymes and redox partners.
WOS关键词STREPTOMYCES-COELICOLOR A3(2) ; ELECTRON-TRANSFER COMPLEX ; 1.7 ANGSTROM RESOLUTION ; PLANT-TYPE FERREDOXINS ; CRYSTAL-STRUCTURE ; IRON-SULFUR ; CORYNEBACTERIUM-GLUTAMICUM ; ESCHERICHIA-COLI ; CYTOCHROME-P450 SYSTEMS ; 3-DIMENSIONAL STRUCTURE
资助项目Shandong Provincial Natural Science Foundation[ZR2017ZB0207] ; Shandong Provincial Natural Science Foundation[ZR2016CB10] ; National Natural Science Foundation of China[81741155] ; National Natural Science Foundation of China[31422002] ; National Natural Science Foundation of China[21472204] ; National Natural Science Foundation of China[31570030] ; Qilu Youth Scholar Startup Funding of Shandong University ; Chinese Academy of Sciences[QYZDB-SSW-SMC042] ; National Postdoctoral Innovative Talent Support Program[BX20180325] ; China Postdoctoral Science Foundation[188229]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000449723900009
出版者AMER CHEMICAL SOC
资助机构Shandong Provincial Natural Science Foundation ; National Natural Science Foundation of China ; Qilu Youth Scholar Startup Funding of Shandong University ; Chinese Academy of Sciences ; National Postdoctoral Innovative Talent Support Program ; China Postdoctoral Science Foundation
源URL[http://ir.qibebt.ac.cn/handle/337004/11019]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Li, Shengying
作者单位1.Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, CAS Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China
3.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China
4.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Wei,Du, Lei,Li, Fengwei,et al. Mechanistic Insights into Interactions between Bacterial Class I P450 Enzymes and Redox Partners[J]. ACS CATALYSIS,2018,8(11):9992-10003.
APA Zhang, Wei.,Du, Lei.,Li, Fengwei.,Zhang, Xingwang.,Qu, Zepeng.,...&Li, Shengying.(2018).Mechanistic Insights into Interactions between Bacterial Class I P450 Enzymes and Redox Partners.ACS CATALYSIS,8(11),9992-10003.
MLA Zhang, Wei,et al."Mechanistic Insights into Interactions between Bacterial Class I P450 Enzymes and Redox Partners".ACS CATALYSIS 8.11(2018):9992-10003.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

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