Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis
文献类型：期刊论文
| 作者 | Jiang Yuanyuan1,2; Li Shengying1,2
|
| 刊名 | CHINESE JOURNAL OF ORGANIC CHEMISTRY
 |
| 出版日期 | 2018-09-25 |
| 卷号 | 38期号:9页码:2307-2323 |
| 关键词 | cytochrome P450 enzyme biosynthesis organic synthesis protein engineering substrate engineering metabolic engineering synthetic biology redox partner |
| ISSN号 | 0253-2786 |
| DOI | 10.6023/cjoc201805055 |
| 英文摘要 | Cytochrome P450 enzymes are widely distributed in nature, which mainly participate in xenobiotics metabolism and natural product biosynthesis. These enzymes are able to recognize various substrates to produce many useful oxidative products through diverse reaction types. P450 enzymes can catalyze selective oxidation of C-H bonds in their substrates under mild conditions. Therefore, a lot of P450 enzymes have been applied in the production of fine chemicals, drugs and chemical intermediates for quite a long time. With the development of protein engineering, redox partner engineering, substrate engineering, metabolic engineering and synthetic biology, it has become possible to obtain the P450 biocatalysts with the desired properties such as high activity, the substrate specificity of interest, and great selectivity to meet the industrial requirements, through rational design and direct evolution of P450 enzymes. Thus, the application scope of P450 enzymes in biosynthesis and organic synthesis has been expanded greatly. The types of reactions that can be catalyzed by P450 enzymes, and the strategies to broaden the reaction scope and to enhance the catalytic efficiency and selectivity are summarized. Finally, the challenges and prospects in the application of cytochrome P450 enzymes in biosynthesis and organic synthesis are discussed. |
| WOS关键词 | CELL BIOTRANSFORMATION SYSTEM ; DIRECTED EVOLUTION ; NATURAL-PRODUCTS ; P450 ENZYMES ; SATURATION MUTAGENESIS ; ALKALOID BIOSYNTHESIS ; MOLECULAR-CLONING ; LIVER-MICROSOMES ; TERPENE CYCLASE ; HYDROXYLATION |
| 资助项目 | Natural Science Foundation of Shandong Province[ZR2017ZB0207] ; National Natural Science Foundation of China[81741115] ; National Natural Science Foundation of China[21472204] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000450205800009 |
| 出版者 | SCIENCE PRESS |
| 资助机构 | Natural Science Foundation of Shandong Province ; National Natural Science Foundation of China |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/11052]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Li Shengying |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang Yuanyuan,Li Shengying. Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis[J]. CHINESE JOURNAL OF ORGANIC CHEMISTRY,2018,38(9):2307-2323. |
| APA | Jiang Yuanyuan,&Li Shengying.(2018).Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis.CHINESE JOURNAL OF ORGANIC CHEMISTRY,38(9),2307-2323. |
| MLA | Jiang Yuanyuan,et al."Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis".CHINESE JOURNAL OF ORGANIC CHEMISTRY 38.9(2018):2307-2323. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。