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Firmicutes-enriched IS1447 represents a group of IS3-family insertion sequences exhibiting unique+1 transcriptional slippage

文献类型：期刊论文

作者Liu, Ya-Jun1,2; Qi, Kuan1,2,3; Zhang, Jie1,2,3,4; Chen, Chao1,2; Cui, Qiu1,2; Feng, Yingang1,2
刊名BIOTECHNOLOGY FOR BIOFUELS
出版日期2018-11-01
卷号11页码:14
关键词Transposable element (TE) Frameshift Lignocellulose Thermophilic Clostridium thermocellum
ISSN号1754-6834
DOI10.1186/s13068-018-1304-8
英文摘要BackgroundBacterial insertion sequences (ISs) are ubiquitous mobile genetic elements that play important roles in genome plasticity, cell adaptability, and function evolution. ISs of various families and subgroups contain significantly diverse molecular features and functional mechanisms that are not fully understood.ResultsIS1447 is a member of the widespread IS3 family and was previously detected to have transposing activity in a typical thermophilic and cellulolytic microorganism Clostridium thermocellum. Phylogenetic analysis showed that IS1447-like elements are widely distributed in Firmicutes and possess unique features in the IS3 family. Therefore, IS1447 may represent a novel subgroup of the IS3 family. Unlike other well-known IS3 subgroups performing programmed -1 translational frameshifting for the expression of the transposase, IS1447 exhibits transcriptional slippage in both the +1 and -1 directions, each with a frequency of similar to 16%, and only +1 slippage results in full-length and functional transposase. The slippage-prone region of IS1447 contains a run of nine A nucleotides following a stem-loop structure in mRNA, but mutagenesis analysis indicated that seven of them are sufficient for the observed slippage. Western blot analysis indicated that IS1447 produces three types of transposases with alternative initiations. Furthermore, the IS1447-subgroup elements are abundant in the genomes of several cellulolytic bacteria.ConclusionOur result indicated that IS1447 represents a new Firmicutes-enriched subgroup of the IS3 family. The characterization of the novel IS3-family member will enrich our understanding of the transposition behavior of IS elements and may provide insight into developing IS-based mutagenesis tools for thermophiles.
WOS关键词CLOSTRIDIUM-THERMOCELLUM ; TRANSPOSABLE ELEMENT ; ESCHERICHIA-COLI ; ETHANOL-PRODUCTION ; GENE-EXPRESSION ; TRANSPOSITION ; IS911 ; DNA ; PROTEIN ; GENOME
资助项目"Transformational Technologies for Clean Energy and Demonstration", Strategic Priority Research Program of the Chinese Academy of Sciences[XDA 21060201] ; National Key Technology Research and Development Program of China[2015BAD15B05] ; National Natural Science Foundation of China[31570029] ; National Natural Science Foundation of China[31670735] ; National Natural Science Foundation of China[31661143023] ; Key Technology Research and Development Program of Shandong[2018GSF1160165] ; Shandong Provincial Natural Science Foundation[ZR2016CB09] ; Major Program of Shandong Provincial Natural Science Foundation[ZR2018ZB0208]
WOS研究方向Biotechnology & Applied Microbiology ; Energy & Fuels
语种英语
WOS记录号WOS:000449116300003
出版者BMC
资助机构"Transformational Technologies for Clean Energy and Demonstration", Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key Technology Research and Development Program of China ; National Natural Science Foundation of China ; Key Technology Research and Development Program of Shandong ; Shandong Provincial Natural Science Foundation ; Major Program of Shandong Provincial Natural Science Foundation
源URL[http://ir.qibebt.ac.cn/handle/337004/11177]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Cui, Qiu; Feng, Yingang
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, CAS Key Lab Biofuels, Qingdao, Peoples R China
2.Dalian Natl Lab Clean Energy, Dalian, Peoples R China
3.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing, Peoples R China
4.Auburn Univ, Dept Biosyst Engn, Auburn, AL 36849 USA
推荐引用方式
GB/T 7714		 Liu, Ya-Jun,Qi, Kuan,Zhang, Jie,et al. Firmicutes-enriched IS1447 represents a group of IS3-family insertion sequences exhibiting unique+1 transcriptional slippage[J]. BIOTECHNOLOGY FOR BIOFUELS,2018,11:14.
APA Liu, Ya-Jun,Qi, Kuan,Zhang, Jie,Chen, Chao,Cui, Qiu,&Feng, Yingang.(2018).Firmicutes-enriched IS1447 represents a group of IS3-family insertion sequences exhibiting unique+1 transcriptional slippage.BIOTECHNOLOGY FOR BIOFUELS,11,14.
MLA Liu, Ya-Jun,et al."Firmicutes-enriched IS1447 represents a group of IS3-family insertion sequences exhibiting unique+1 transcriptional slippage".BIOTECHNOLOGY FOR BIOFUELS 11(2018):14.

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来源：青岛生物能源与过程研究所

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