The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine
文献类型：期刊论文
| 作者 | Bao, Zixian; Sun, Yue; Rai, Kamal; Peng, Xinying; Wang, Shilu; Nian, Rui; Xian, Mo
|
| 刊名 | BIOMATERIALS SCIENCE
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 6期号:11页码:3042-3052 |
| ISSN号 | 2047-4830 |
| DOI | 10.1039/c8bm00675j |
| 英文摘要 | Collagen has been widely documented as one of the most promising and competitive biomaterials for tissue engineering and medical applications. However, the properties of collagen differ from one source to another. In the present study, type I collagen (COL-I) was extracted and purified from the skins of Japanese sea bass (Lateolabrax japonicus) and Nile tilapia (Oreochromis niloticus). Ultraviolet (UV) spectroscopy, Fourier transform infrared spectroscopy (FTIR) and SDS-PAGE were performed to characterize both COL-Is. The denaturing temperature of bass collagen (BC) was observed to be 27.2 degrees C, and 35.3 degrees C for tilapia collagen (TC). The content of hydroxyproline was 13.4% in TC, which was similar to that in porcine collagen (PC, 13.6%) and higher than that in BC (10.3%), while the content of cysteine in TC (0.87%) was significantly higher than that in PC (0.04%) and BC (0.35%). After incubation at different temperatures for 9 h, more degraded collagen bands appeared in the BC hydrogel (BCH) group than in the TC hydrogel (TCH) group, indicating that TCH exhibited better thermal stability than BCH. The thermal stabilities of TCH and PC hydrogel (PCH) were similar. The compressive stress of TCH was up to 0.099 MPa, while it was 0.047 MPa for BCH and 0.003 MPa for PCH. These results demonstrated that the content of amino acids (especially hydroxyproline and cysteine) has a synergistic effect on the thermal and mechanical properties of BCH, TCH and PCH, which would be an indicator of the thermal and mechanical properties of collagen hydrogels in future studies. |
| WOS关键词 | PEPSIN-SOLUBILIZED COLLAGEN ; FISH SCALE COLLAGEN ; ACID-SOLUBLE COLLAGEN ; ROHU LABEO-ROHITA ; FIBRIL FORMATION ; I COLLAGEN ; OREOCHROMIS-NILOTICAS ; PHYSICAL-PROPERTIES ; PAGRUS-MAJOR ; SWIM BLADDER |
| 资助项目 | Shandong Provincial Natural Science Foundation, China[ZR2018BC027] ; National Natural Science Foundation of China[21676286] ; Primary Research Development Plan of Shandong Province[2016GSF121006] ; China Postdoctoral Science Foundation[2017M622302] |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000448822800023 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | Shandong Provincial Natural Science Foundation, China ; National Natural Science Foundation of China ; Primary Research Development Plan of Shandong Province ; China Postdoctoral Science Foundation |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/11198]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Nian, Rui; Xian, Mo |
| 作者单位 | Chinese Acad Sci, CAS Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, Zixian,Sun, Yue,Rai, Kamal,et al. The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine[J]. BIOMATERIALS SCIENCE,2018,6(11):3042-3052. |
| APA | Bao, Zixian.,Sun, Yue.,Rai, Kamal.,Peng, Xinying.,Wang, Shilu.,...&Xian, Mo.(2018).The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine.BIOMATERIALS SCIENCE,6(11),3042-3052. |
| MLA | Bao, Zixian,et al."The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine".BIOMATERIALS SCIENCE 6.11(2018):3042-3052. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。