Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification
文献类型：期刊论文
| 作者 | Wang, Yanli1; Chen, Quan2; Xian, Mo2; Nian, Rui2; Xu, Fei1 |
| 刊名 | AMB EXPRESS
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 8 |
| 关键词 | Monoclonal Antibody Electronegative Multimodal Non-affinity Purification Cost-savings |
| ISSN号 | 2191-0855 |
| DOI | 10.1186/s13568-018-0622-3 |
| 文献子类 | Article |
| 英文摘要 | In recent studies, electronegative multimodal chromatography with Eshmuno HCX was demonstrated to be a highly promising recovery step for direct immunoglobulin G (IgG) capture from undiluted cell culture fluid. In this study, the binding properties of HCX to IgG at different pH/salt combinations were systematically studied, and its purification performance was significantly enhanced by lowering the washing pH and conductivity after high capacity binding of IgG under its optimal conditions. A single polishing step gave an end-product with non-histone host cell protein (nh-HCP) below 1 ppm, DNA less than 1 ppb, which aggregates less than 0.5% and an overall IgG recovery of 86.2%. The whole non-affinity chromatography based two-column-step process supports direct feed loading without buffer adjustment, thus extraordinarily boosting the overall productivity and cost-savings. |
| WOS关键词 | CATION-EXCHANGE CHROMATOGRAPHY ; HYDROPHOBIC INTERACTION CHROMATOGRAPHY ; PROTEIN-A CHROMATOGRAPHY ; CELL-CULTURE FLUID ; MONOCLONAL-ANTIBODIES ; VOID-EXCLUSION ; CHROMATIN ; PERFORMANCE ; PH ; CLEARANCE |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000433978500004 |
| 出版者 | BIOMED CENTRAL LTD |
| 资助机构 | National Natural Science Foundation of China(21676286) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/11391]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Nian, Rui; Xu, Fei |
| 作者单位 | 1.Jilin Univ, Coll Life Sci, Changchun, Jilin, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yanli,Chen, Quan,Xian, Mo,et al. Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification[J]. AMB EXPRESS,2018,8. |
| APA | Wang, Yanli,Chen, Quan,Xian, Mo,Nian, Rui,&Xu, Fei.(2018).Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification.AMB EXPRESS,8. |
| MLA | Wang, Yanli,et al."Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification".AMB EXPRESS 8(2018). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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