MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions
文献类型：期刊论文
| 作者 | Sun, Qing1; Liu, Xiaogang1; Yang, Juan1; Liu, Wenwen; Du, Qingguo1; Wang, Hongqiu1; Fu, Chunxiang2,3; Li, Wen-Xue1 |
| 刊名 | MOLECULAR PLANT
 |
| 出版日期 | 2018-06-04 |
| 卷号 | 11期号:6页码:806-814 |
| ISSN号 | 1674-2052 |
| 关键词 | Maize Nitrogen Zmmir528 Zmlacs Lodging Resistance |
| DOI | 10.1016/j.molp.2018.03.013 |
| 文献子类 | Article |
| 英文摘要 | Lodging under nitrogen (N)-luxury conditions substantially reduces crop yield and seed quality. However, the molecular mechanisms of plant lodging resistance remain largely unclear, especially in maize. We report here that the expression of ZmmiR528, a monocot-specific microRNA, is induced by N luxury but reduced by N deficiency. We show by the thioacidolysis and acetyl bromide analysis that N luxury significantly reduces the generation of H, G, and S monomers of the lignin as well as its total content in maize shoots. We further demonstrate that ZmLACCASE3 (ZmLAC3) and ZmLACCASE5 (ZmLAC5), which encode the copper-containing laccases, are the targets of ZmmiR528. In situ hybridization showed that ZmmiR528 is mainly expressed in maize vascular tissues. Knockdown of ZmmiR528 or overexpression of ZmLAC3 significantly increased the lignin content and rind penetrometer resistance of maize stems. In contrast, transgenic maize plants overexpressing ZmmiR528 had reduced lignin content and rind penetrometer resistance and were prone to lodging under N-luxury conditions. RNA-sequencing analysis revealed that ZmPAL7 and ZmPAL8 are upregulated in transgenic maize lines downregulating ZmmiR528. Under N-luxury conditions, the expression levels of ZmPALs were much higher in ZmmiR528-knockdown lines than in the wild type and transgenic maize lines overexpressing ZmmiR528. Taken together, these results indicate that, by regulating the expression ofZmLAC3 and ZmLAC5, ZmmiR528 affects maize lodging resistance under N-luxury conditions. |
| WOS关键词 | ARABIDOPSIS-THALIANA ROOTS ; CELL-WALL LIGNIFICATION ; TRANSCRIPTION FACTORS ; NITRATE RESPONSE ; GENE-EXPRESSION ; LACCASE ; PLANTS ; SYSTEMS ; FERTILIZATION ; MANAGEMENT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:000434429000005 |
| 资助机构 | National Key Research and Development Program of China(2016YFD0100701) ; Ministry of Agriculture of China for Transgenic Research(2018ZX0800988B) ; Agricultural Science and Technology Innovation Program of CAAS |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/11409]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Li, Wen-Xue |
| 作者单位 | 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Engn Lab Crop Mol Breeding, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Qingdao Engn Res Ctr Biomass Resources & Environm, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Qing,Liu, Xiaogang,Yang, Juan,et al. MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions[J]. MOLECULAR PLANT,2018,11(6):806-814. |
| APA | Sun, Qing.,Liu, Xiaogang.,Yang, Juan.,Liu, Wenwen.,Du, Qingguo.,...&Li, Wen-Xue.(2018).MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions.MOLECULAR PLANT,11(6),806-814. |
| MLA | Sun, Qing,et al."MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions".MOLECULAR PLANT 11.6(2018):806-814. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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